The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus

Danxia Ke; Qing Fang; Chunfen Chen; Hui Zhu; Tao Chen; Xiaojun Chang; Songli Yuan; Heng Kang; Lian Ma; Zonglie Hong; Zhongming Zhang

doi:10.1104/pp.112.197269

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The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus

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The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus

Danxia Ke, Qing Fang, Chunfen Chen, Hui Zhu, Tao Chen, Xiaojun Chang, Songli Yuan, Heng Kang, Lian Ma, Zonglie Hong, …

Plant physiology (Bethesda), Vol.159(1), pp.131-143

05/2012

DOI: https://doi.org/10.1104/pp.112.197269

Handle:

https://hdl.handle.net/2376/104093

PMID: 22434040

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Abstract

Genes, Plant

Molecular Sequence Data

Plant Roots - genetics

Plant Diseases - microbiology

Plant Epidermis - genetics

Phylogeny

Plant Epidermis - metabolism

RNA Interference

Gene Expression Regulation, Plant

Plasmids - genetics

Agrobacterium - metabolism

Cell Membrane - metabolism

Plant Proteins - metabolism

Protein Structure, Tertiary

Symbiosis

Amino Acid Sequence

Green Fluorescent Proteins - metabolism

Plant Roots - metabolism

Mesorhizobium - pathogenicity

Plants, Genetically Modified - genetics

Tobacco - metabolism

Loteae - metabolism

Loteae - microbiology

Monomeric GTP-Binding Proteins - genetics

Plasmids - metabolism

Protein Interaction Mapping

Plant Proteins - genetics

Two-Hybrid System Techniques

GTP Phosphohydrolases - metabolism

Plants, Genetically Modified - metabolism

Plants, Genetically Modified - microbiology

Monomeric GTP-Binding Proteins - metabolism

Mesorhizobium - metabolism

Plant Root Nodulation

Plant Vascular Bundle - metabolism

Agrobacterium - genetics

Tobacco - microbiology

Enzyme Activation

Photosynthesis

Loteae - genetics

Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and is required for the early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as an NFR5-interacting protein. The amino acid sequence of this Rho-like GTPase is closest to the Arabidopsis (Arabidopsis thaliana) ROP6 and Medicago truncatula ROP6 and was designated as LjROP6. The interaction between Rop6 and NFR5 occurred both in vitro and in planta. No interaction between Rop6 and NFR1 was observed. Green fluorescent protein-tagged ROP6 was localized at the plasma membrane and cytoplasm. The interaction between ROP6 and NFR5 appeared to take place at the plasma membrane. The expression of the ROP6 gene could be detected in vascular tissues of Lotus roots. After inoculation with Mesorhizobium loti, elevated levels of ROP6 expression were found in the root hairs, root tips, vascular bundles of roots, nodule primordia, and young nodules. In transgenic hairy roots expressing ROP6 RNA interference constructs, Rhizobium entry into the root hairs did not appear to be affected, but infection thread growth through the root cortex were severely inhibited, resulting in the development of fewer nodules per plant. These data demonstrate a role of ROP6 as a positive regulator of infection thread formation and nodulation in L. japonicus.

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Title: The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus
Creators: Danxia Ke - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Qing Fang
Chunfen Chen
Hui Zhu
Tao Chen
Xiaojun Chang
Songli Yuan
Heng Kang
Lian Ma
Zonglie Hong
Zhongming Zhang
Publication Details: Plant physiology (Bethesda), Vol.159(1), pp.131-143
Academic Unit: Center for Reproductive Biology; Crop and Soil Sciences, Department of
Publisher: United States
Identifiers: 99900546997401842
Language: English
Resource Type: Journal article