The streptomycin-sulfadiazine-tetracycline antimicrobial resistance element of calf-adapted Escherichia coli is widely distributed among isolates from Washington state cattle

Artashes R Khachatryan; Thomas E Besser; Douglas R Call

doi:10.1128/AEM.01534-07

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The streptomycin-sulfadiazine-tetracycline antimicrobial resistance element of calf-adapted Escherichia coli is widely distributed among isolates from Washington state cattle

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The streptomycin-sulfadiazine-tetracycline antimicrobial resistance element of calf-adapted Escherichia coli is widely distributed among isolates from Washington state cattle

Artashes R Khachatryan, Thomas E Besser and Douglas R Call

Applied and environmental microbiology, Vol.74(2), pp.391-395

01/2008

DOI: https://doi.org/10.1128/AEM.01534-07

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https://hdl.handle.net/2376/109445

PMCID: PMC2223241

PMID: 18039823

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Abstract

Animals, Newborn

Streptomycin - pharmacology

Escherichia coli - drug effects

Tetracycline - pharmacology

Gastrointestinal Tract - microbiology

Sulfadiazine - pharmacology

Electrophoresis, Gel, Pulsed-Field

Sequence Analysis, DNA

Washington

Microbial Sensitivity Tests

Phenotype

Animals

Cattle

Escherichia coli - genetics

Escherichia coli - isolation & purification

Chloramphenicol - pharmacology

Nalidixic Acid - pharmacology

Ampicillin - pharmacology

Anti-Bacterial Agents - pharmacology

Models, Genetic

Drug Resistance, Multiple, Bacterial - genetics

Association of specific antimicrobial resistance patterns with unrelated selective traits has long been implicated in the maintenance of antimicrobial resistance in a population. Previously we demonstrated that Escherichia coli strains with a specific resistance pattern (resistant to streptomycin, sulfadiazine, and tetracycline [SSuT]) have a selective advantage in dairy calf intestinal environments and in the presence of a milk supplement commonly fed to the calves. In the present study we identified the sequence of the genetic element that confers the SSuT phenotype and show that this element is present in a genetically diverse group of E. coli isolates, as assessed by macrorestriction digestion and pulsed-field gel electrophoresis. This element was also found in E. coli isolates from 18 different cattle farms in Washington State. Using in vitro competition experiments we further demonstrated that SSuT strains from 17 of 18 farms were able to outcompete pansusceptible strains. In a separate set of experiments, we were able to transfer the antimicrobial resistance phenotype by electroporation to a laboratory strain of E. coli (DH10B), making that new strain more competitive during in vitro competition with the parental DH10B strain. These data indicate that a relatively large genetic element conferring the SSuT phenotype is widely distributed in E. coli from cattle in Washington State. Furthermore, our results indicate that this element is responsible for maintenance of these traits owing to linkage to genetic traits that confer a selective advantage in the intestinal lumens of dairy calves.

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Title: The streptomycin-sulfadiazine-tetracycline antimicrobial resistance element of calf-adapted Escherichia coli is widely distributed among isolates from Washington state cattle
Creators: Artashes R Khachatryan - Dept. of Veterinary Microbiology & Pathology, 402 Bustad Hall, Pullman, WA 99164-7040, USA
Thomas E Besser
Douglas R Call
Publication Details: Applied and environmental microbiology, Vol.74(2), pp.391-395
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: United States
Identifiers: 99900547159501842
Language: English
Resource Type: Journal article