Journal article
The use of Fourier transform infrared spectroscopy to differentiate Escherichia coli O157:H7 from other bacteria inoculated into apple juice
Food microbiology, Vol.23(2), pp.162-168
2006
Handle:
https://hdl.handle.net/2376/106870
PMID: 16943000
Abstract
Fourier transform infrared spectroscopy (FT-IR) can discriminate
Escherichia coli O157:H7 ATCC 35150 from other bacteria:
E. coli ATCC 25522,
Bacillus cereus ATCC 10876, and
Listeria innocua ATCC 51742 inoculated in to apple juice. Spectra of bacterial suspensions (ca. 10
9 cfu/ml in 0.9% NaCl) on Anodisc (aluminum oxide) filters were tested. Unique FT-IR vibrational combination bands from mid-IR active components of bacterial cells are present in the “fingerprint region” at wavenumbers between 1500 and 800
cm
−1. Principal component analysis (PCA) revealed clear segregations between different bacterial strains. Also, soft independent modeling of class analogy (SIMCA) correctly classified
E. coli O157:H7 ATCC 35150 from
E. coli ATCC 25522 at an 82% confidence level; whereas a 77% confidence level was obtained when using SIMCA to classify
E. coli O157:H7 from three other bacterial strains.
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Details
- Title
- The use of Fourier transform infrared spectroscopy to differentiate Escherichia coli O157:H7 from other bacteria inoculated into apple juice
- Creators
- Murad A Al-Holy - Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, Zarqa-JordanMengshi Lin - Department of Food Science and Human Nutrition, Washington State University, Box 646376, Pullman, WA 99164-6373, USAAnna G Cavinato - Chemistry and Biochemistry Program, Eastern Oregon University, One University Blvd., La Grande, OR 97850-2899, USABarbara A Rasco - Department of Food Science and Human Nutrition, Washington State University, Box 646376, Pullman, WA 99164-6373, USA
- Publication Details
- Food microbiology, Vol.23(2), pp.162-168
- Academic Unit
- Food Science, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546742401842
- Language
- English
- Resource Type
- Journal article