Journal article
The virtual element in proximal promoter of porcine myostatin is regulated by myocyte enhancer factor 2C
Biochemical and biophysical research communications, Vol.419(2), pp.175-181
03/09/2012
Handle:
https://hdl.handle.net/2376/114997
PMID: 22342668
Abstract
► A enhancer site located at −206 to −189 in porcine myostatin promoter is identified. ► The F1 site with A/T rich residues is crucial for porcine myostatin expression. ► MEF2C physically interacts with F1 site and upregulates the myostatin expression.
Myostatin (myostatin) is a member of the transforming growth factor-β superfamily. In this study, we investigated the transcriptional regulation of porcine myostatin expression in C2C12 cells. Sequence analysis of 2kb of the porcine myostatin gene upstream region revealed that it contains 16 E-box motifs. Using a serial deletion strategy, we identified a vital enhancer region between nucleotides −218 and −137 in promoter region of porcine myostatin. Gel-shift assay and deletion analysis result showed F1 site, from nucleotides −206 to −189, was the key element to produce the promoter/nucleus extract complex, which directly interact with transcriptional factor MEF2C. Overexpression of MEF2C increased myostatin promoter activity in dose dependent manner; and whereas introducing of small interfering RNA to MEF2C produced the opposite effect in both myoblasts and myotubes. When endogenous MEF2C was knocked down, the complex of protein/F1 was attenuated, and myostatin mRNA and protein level was also decreased. Thus, we propose that MEF2C could modulate and restrain myogenesis by myostatin activation and myostatin-dependent gene processing in porcine.
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Details
- Title
- The virtual element in proximal promoter of porcine myostatin is regulated by myocyte enhancer factor 2C
- Creators
- Jia LiJie DengShan YuJunlin ZhangDe ChengHuayan Wang
- Publication Details
- Biochemical and biophysical research communications, Vol.419(2), pp.175-181
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547999601842
- Language
- English
- Resource Type
- Journal article