Journal article
The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathion
Nanoscale, Vol.5(3), pp.1121-1126
02/07/2013
Handle:
https://hdl.handle.net/2376/105506
PMID: 23280070
Abstract
A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe(3)O(4) core was synthesized using cysteamine (SH-NH(2)) as linker, and characterized by TEM, XPS, UV and electrochemistry. Then a hydrolase biosensor, based on self-assembly of methyl parathion hydrolase (MPH) on the Fe(3)O(4)@Au nanocomposite, was developed for sensitive and selective detection of the organophosphorus pesticide (OP) methyl parathion. The magnetic nanocomposite provides an easy way to construct the enzyme biosensor by simply exerting an external magnetic field, and also provides a simple way to renew the electrode surface by removing the magnet. Unlike inhibition-based enzyme biosensors, the hydrolase is not poisoned by OPs and thus is reusable for continuous measurement. AuNPs not only provide a large surface area, high loading efficiency and fast electron transfer, but also stabilize the enzyme through electrostatic interactions. The MPH biosensor shows rapid response and high selectivity for detection of methyl parathion, with a linear range from 0.5 to 1000 ng mL(-1) and a detection limit of 0.1 ng mL(-1). It also shows acceptable reproducibility and stability. The simplicity and ease of operation of the proposed method has great potential for on-site detection of P-S containing pesticides and provides a promising strategy to construct a robust biosensor.
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Details
- Title
- The vital function of Fe3O4@Au nanocomposites for hydrolase biosensor design and its application in detection of methyl parathion
- Creators
- Yuting Zhao - Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaWeiying ZhangYuehe LinDan Du
- Publication Details
- Nanoscale, Vol.5(3), pp.1121-1126
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- England
- Grant note
- U01 NS058161-01 / NINDS NIH HHS U01 NS058161 / NINDS NIH HHS
- Identifiers
- 99900547001701842
- Language
- English
- Resource Type
- Journal article