Journal article
The western red cedar (Thuja plicata) 8-8′ DIRIGENT family displays diverse expression patterns and conserved monolignol coupling specificity
Plant molecular biology, Vol.49(2), pp.199-214
05/2002
Handle:
https://hdl.handle.net/2376/110588
PMID: 11999375
Abstract
The isolation and characterization of a multigene family of the first class of dirigent proteins (namely that mainly involved in 8-8′ coupling leading to (+)-pinoresinol in this case) is reported, this comprising of nine western red cedar (Thuja plicata) DIRIGENT genes (DIR1–9) of 72–99.5% identity to each other. Their corresponding cDNA clones had coding regions for 180–183 amino acids with each having a predicted molecular mass of ca. 20 kDa including the signal peptide. Real time-PCR established that the DIRIGENT isovariants were differentially expressed during growth and development of T. plicata (P<0.05). The phylogenetic relationships and the rates and patterns of nucleotide substitution suggest that the DIRIGENT gene may have evolved via paralogous expansion at an early stage of vascular plant diversification. Thereafter, western red cedar paralogues have maintained an high homogeneity presumably via a concerted evolutionary mode. This, in turn, is assumed to be the driving force for the differential formation of 8-8′-linked pinoresinol derived (poly)lignans in the needles, stems, bark and branches, as well as for massive accumulation of 8-8′-linked plicatic acid-derived (poly)lignans in heartwood.
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Details
- Title
- The western red cedar (Thuja plicata) 8-8′ DIRIGENT family displays diverse expression patterns and conserved monolignol coupling specificity
- Creators
- Myoung Kim - Institute of Biological Chemistry 467 Clark Hall, Washington State University Pullman WA 99164-6340 USAJae-Heung Jeon - Institute of Biological Chemistry 467 Clark Hall, Washington State University Pullman WA 99164-6340 USAMasayuki Fujita - Institute of Biological Chemistry 467 Clark Hall, Washington State University Pullman WA 99164-6340 USALaurence Davin - Institute of Biological Chemistry 467 Clark Hall, Washington State University Pullman WA 99164-6340 USANorman Lewis - Institute of Biological Chemistry 467 Clark Hall, Washington State University Pullman WA 99164-6340 USA
- Publication Details
- Plant molecular biology, Vol.49(2), pp.199-214
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547614401842
- Language
- English
- Resource Type
- Journal article