Journal article
Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos
Plant cell reports, Vol.15(3), pp.164-169
12/1995
Handle:
https://hdl.handle.net/2376/105139
PMID: 24185768
Abstract
Protoplasts of 10 cultivars of V. faba were isolated from etiolated shoot-tips and tested for their regeneration capacity. After purification, protoplasts were embedded in sodium alginate and cultivated in the medium of Kao and Michayluk (1975) containing 0.5 mg·1−1 of each 2,4-dichlorophenoxyacetic acid, naphthylacetic acid and 6-benzylaminopurine. Depending on cultivar, division frequencies of up to 40% were obtained. Six weeks after embedding, protoplast-derived calluses were transferred to Gelrite-solidified media with different combinations of growth regulators. A two step protocol (auxin high/low) was tested for its ability to induce somatic embryogenesis. The formation of globular structures was observed, but no embryo formation could be achieved. In contrast, cultivation of protocalluses on medium supplemented with thidiazuron resulted in shoot development in cultivar Mythos. To generate mature plants, the shoots were grafted onto young seedlings. In order to optimize the in vitro-conditions, different concentrations of thidiazuron alone or in combination with naphthylacetic acid were tested, showing that an increase of thidiazuron and the addition of naphthylacetic acid positively affects both the viability of protocalluses and the regeneration frequency.
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Details
- Title
- Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos
- Creators
- Mechthild Tegeder - 0000 0000 9116 4836 grid.14095.39 Freie Universität Berlin, Institut für Angewandte Genetik Albrecht-Thaer-Weg 6 14195 Berlin GermanyDorothea Gebhardt - 0000 0000 9116 4836 grid.14095.39 Freie Universität Berlin, Institut für Angewandte Genetik Albrecht-Thaer-Weg 6 14195 Berlin GermanyOtto Schieder - 0000 0000 9116 4836 grid.14095.39 Freie Universität Berlin, Institut für Angewandte Genetik Albrecht-Thaer-Weg 6 14195 Berlin GermanyThomas Pickardt - 0000 0000 9116 4836 grid.14095.39 Freie Universität Berlin, Institut für Angewandte Genetik Albrecht-Thaer-Weg 6 14195 Berlin Germany
- Publication Details
- Plant cell reports, Vol.15(3), pp.164-169
- Academic Unit
- Biological Sciences, School of
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Identifiers
- 99900546879401842
- Language
- English
- Resource Type
- Journal article