Journal article
Tight correlation between inhibition of DNA repair in vitro and transcription factor IIIA binding in a 5S ribosomal RNA gene
The EMBO journal, Vol.18(5), pp.1387-1396
03/01/1999
Handle:
https://hdl.handle.net/2376/108150
PMCID: PMC1171228
PMID: 10064604
Abstract
UV‐induced photoproducts (cyclobutane pyrimidine dimers, CPDs) in DNA are removed by nucleotide excision repair (NER), and the presence of transcription factors on DNA can restrict the accessibility of NER enzymes. We have investigatigated the modulation of NER in a gene promoter using the Xenopus transcription factor IIIA (TFIIIA)–5S rDNA complex and Xenopus oocyte nuclear extracts. TFIIIA alters CPD formation primarily in the transcribed strand of the 50 bp internal control region (ICR) of 5S rDNA. During NER in vitro, CPD removal is reduced at most sites in both strands of the ICR when TFIIIA is bound. Efficient repair occurs just outside the TFIIIA‐binding site (within 10 bp), and in the absence of 5S rRNA transcription. Interestingly, three CPD sites within the ICR [+56, +75 (transcribed strand) and +73 (non‐transcribed strand)] are repaired rapidly when TFIIIA is bound, while CPDs within ∼5 bases of these sites are repaired much more slowly. CPDs at these three sites may partially displace TFIIIA, thereby enabling rapid repair. However, TFIIIA is not completely displaced during NER, at least at sites outside the ICR, even though the NER complex could be sterically hindered by TFIIIA. Such inefficient repair of transcription factor binding sites could increase mutation frequency in regulatory regions of genes.
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Details
- Title
- Tight correlation between inhibition of DNA repair in vitro and transcription factor IIIA binding in a 5S ribosomal RNA gene
- Creators
- Antonio Conconi - Department of Biochemistry and Biophysics, Washington State UniversityXiaoqi Liu - Department of Biochemistry and Biophysics, Washington State UniversityLilia Koriazova - Molecular Biosciences Department, Pacific Northwest National Laboratory, P7‐56, Box 999Eric J Ackerman - Molecular Biosciences Department, Pacific Northwest National Laboratory, P7‐56, Box 999Michael J Smerdon - Department of Biochemistry and Biophysics, Washington State University
- Publication Details
- The EMBO journal, Vol.18(5), pp.1387-1396
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- John Wiley & Sons, Ltd; Chichester, UK
- Number of pages
- 10
- Identifiers
- 99900546937801842
- Language
- English
- Resource Type
- Journal article