Time-of-day differences in dopamine clearance in the rat medial prefrontal cortex and nucleus accumbens

Evan P Sleipness; Heiko T Jansen; James O Schenk; Barbara A Sorg

doi:10.1002/syn.20552

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Time-of-day differences in dopamine clearance in the rat medial prefrontal cortex and nucleus accumbens

Journal article

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Time-of-day differences in dopamine clearance in the rat medial prefrontal cortex and nucleus accumbens

Evan P Sleipness, Heiko T Jansen, James O Schenk and Barbara A Sorg

Synapse (New York, N.Y.), Vol.62(12), pp.877-885

12/2008

DOI: https://doi.org/10.1002/syn.20552

Handle:

https://hdl.handle.net/2376/116178

PMCID: PMC3031299

PMID: 18792987

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Abstract

Nucleus Accumbens - metabolism

Rats

Male

Circadian Rhythm - physiology

Rats, Sprague-Dawley

Metabolic Clearance Rate - physiology

Activity Cycles - physiology

Cocaine - pharmacology

Animals

Prefrontal Cortex - drug effects

Prefrontal Cortex - metabolism

Metabolic Clearance Rate - drug effects

Nucleus Accumbens - drug effects

Circadian Rhythm - drug effects

Dopamine - metabolism

Circadian rhythms influence cocaine-seeking behavior in rats, and this behavior may be mediated by variability in the rate of extracellular dopamine clearance across the day:night cycle. We used rotating disk electrode voltammetry to examine dopamine clearance and inhibition of clearance by cocaine in the rat medial prefrontal cortex (mPFC) and nucleus accumbens (NAc). Rats were housed under light:dark conditions (LD, 12 h:12 h) or in constant darkness (DD), the latter given just prior to the day of sacrifice. Tissue was collected at 4-h intervals under LD and DD conditions. Under LD, dopamine clearance in both brain regions was greatest at 4h after lights on. Under DD, there was a blunted but still rhythmic pattern of dopamine clearance across the 24-h cycle. Cocaine-induced inhibition of dopamine clearance in the mPFC was not different across the day:night cycle in rats under LD. Paradoxically, under DD, dopamine clearance in the mPFC was enhanced by cocaine at ZT16, 4 h into the subjective night, and only minimally inhibited at other times. In the NAc, cocaine inhibition of dopamine clearance was lowest at ZT4 under LD, and did not vary under DD. We conclude that dopamine clearance varies both in a diurnal and possibly in a circadian manner in the mPFC, and in a diurnal manner in the NAc. These results indicate that light itself may be used to manipulate molecules implicated in drug addiction.

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Title: Time-of-day differences in dopamine clearance in the rat medial prefrontal cortex and nucleus accumbens
Creators: Evan P Sleipness - Program in Neuroscience, Washington State University, Pullman, Washington 99164, USA
Heiko T Jansen
James O Schenk
Barbara A Sorg
Publication Details: Synapse (New York, N.Y.), Vol.62(12), pp.877-885
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: United States
Grant note: R01 DA023202 / NIDA NIH HHS R01 DA023202-02 / NIDA NIH HHS DA023202 / NIDA NIH HHS
Identifiers: 99900547909501842
Language: English
Resource Type: Journal article