Time of day differences in the number of cytokine-, neurotrophin- and NeuN-immunoreactive cells in the rat somatosensory or visual cortex

Krista Hight; Heather Hallett; Lynn Churchill; Alok De; Andrea Boucher; James M Krueger

doi:10.1016/j.brainres.2010.04.012

Back

Time of day differences in the number of cytokine-, neurotrophin- and NeuN-immunoreactive cells in the rat somatosensory or visual cortex

Journal article

Open access

Peer reviewed

Time of day differences in the number of cytokine-, neurotrophin- and NeuN-immunoreactive cells in the rat somatosensory or visual cortex

Krista Hight, Heather Hallett, Lynn Churchill, Alok De, Andrea Boucher and James M Krueger

Brain research, Vol.1337, pp.32-40

2010

DOI: https://doi.org/10.1016/j.brainres.2010.04.012

Handle:

https://hdl.handle.net/2376/101247

PMCID: PMC2892412

PMID: 20398636

Files and links (1)

url

https://doi.org/10.1016/j.brainres.2010.04.012View

Published (Version of record) Open

Abstract

Glial–neuronal interactions

Sleep

Cytokines

Astrocytes

Use-dependent

Whisker

Sensory input to different cortical areas differentially varies across the light–dark cycle and likely is responsible, in part, for activity-dependent changes in time-of-day differences in protein expression such as Fos. In this study we investigate time-of-day differences between dark (just before light onset) and light (just before dark onset) for the number of immunoreactive (IR) neurons that stained for tumor necrosis factor alpha (TNFα), interleukin-1β (IL1β), nerve growth factor (NGF), the neuronal nuclear protein (NeuN) and Fos in the rat somatosensory cortex (Sctx) and visual cortex (Vctx). Additionally, astrocyte IL1β-IR in the Sctx and Vctx was determined. TNFα and IL1β, as well as the immediate early gene protein Fos, were higher at the end of the dark phase (2300 h) compared to values obtained at the end of the light phase (1100 h) in the Sctx and Vctx. IL1β-IR in Sctx and Vctx astrocytes was higher at 2300 h than that observed at 1100 h. . In contrast, the number of NGF-IR neurons was higher in the Vctx than in the Sctx but did not differ in time. However, the density of the NGF-IR neurons in layer V was greater at 2300 h in the Sctx than at 1100 h. NeuN-IR was higher at 2300 h in the Sctx but was lower at this time in the Vctx compared to 1100 h. These data demonstrate that expressions of the molecules examined are dependent on activity, the sleep-wake cycle and brain location. These factors interact to modulate time-of-day expression.

Metrics

5 Record Views

Details

Title: Time of day differences in the number of cytokine-, neurotrophin- and NeuN-immunoreactive cells in the rat somatosensory or visual cortex
Creators: Krista Hight - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
Heather Hallett - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
Lynn Churchill - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
Alok De - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
Andrea Boucher - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
James M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA
Publication Details: Brain research, Vol.1337, pp.32-40
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: Elsevier B.V
Identifiers: 99900546660301842
Language: English
Resource Type: Journal article