Journal article
Time-of-day regulates subcellular trafficking, tripartite synaptic localization and polyadenylation of the astrocytic Fabp7 mRNA
The Journal of neuroscience, Vol.32(4), pp.1383-1394
01/25/2012
Handle:
https://hdl.handle.net/2376/109146
PMCID: PMC3564590
PMID: 22279223
Abstract
The astrocyte brain fatty acid binding protein (Fabp7) has previously been shown to have a coordinated diurnal regulation of mRNA and protein throughout mouse brain, and an age-dependent decline in protein expression within synaptoneurosomal fractions. Mechanisms that control time-of-day changes in expression and trafficking Fabp7 to the perisynaptic process are not known. In this study, we confirmed an enrichment of Fabp7 mRNA and protein in the astrocytic perisynaptic compartment, and observed a diurnal change in the intracellular distribution of Fabp7 mRNA in molecular layers of hippocampus. Northern blotting revealed a coordinated time-of-day dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain. Cytoplasmic polyadenylation element-(CPE-) binding protein (CPEB1) regulates subcellular trafficking and translation of synaptic plasticity-related mRNAs. Here we show that Fabp7 mRNA co-immunoprecipitated with CPEB1 from primary mouse astrocyte extracts, and its 3′UTR contains phylogenetically conserved CPEs capable of regulating translation of reporter mRNAs during
Xenopus
oocyte maturation. Given that Fabp7 expression is confined to astrocytes and neural progenitors in adult mouse brain, the synchronized cycling pattern of Fabp7 mRNA is therefore novel of known CPE-regulated transcripts. These results implicate circadian, sleep and/or metabolic control of CPEB-mediated subcellular trafficking and localized translation of Fabp7 mRNA in the tripartite synapse of mammalian brain.
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Details
- Title
- Time-of-day regulates subcellular trafficking, tripartite synaptic localization and polyadenylation of the astrocytic Fabp7 mRNA
- Creators
- Jason R Gerstner - Center for Sleep and Circadian Neurobiology, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104William M Vanderheyden - Department of Anatomy and Neurobiology, Washington University Medical School, Saint Louis, MO, 63110Timothy LaVaute - Waisman Center, University of Wisconsin, Madison, WI, 53706Cara J Westmark - Waisman Center, University of Wisconsin, Madison, WI, 53706Labib Rouhana - Department of Biochemistry, University of Wisconsin, Madison, WI, 53706Allan I Pack - Center for Sleep and Circadian Neurobiology, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104Marv Wickens - Department of Biochemistry, University of Wisconsin, Madison, WI, 53706Charles F Landry - Scarab Genomics, LLC, Madison, WI, 53713
- Publication Details
- The Journal of neuroscience, Vol.32(4), pp.1383-1394
- Academic Unit
- Biomedical Sciences, Department of
- Grant note
- R37 GM031892-25 || GM / National Institute of General Medical Sciences : NIGMS
- Identifiers
- 99900547009801842
- Language
- English
- Resource Type
- Journal article