Journal article
Tissue culture of sockeye salmon intestine: functional response of Na+-K+-ATPase to cortisol
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.288(6), pp.R1598-1605
06/2005
Handle:
https://hdl.handle.net/2376/107123
PMID: 15695320
Abstract
A method to culture tissue explants of the intestine from freshwater-adapted sockeye salmon (Oncorhynchus nerka) was developed to assess possible direct effects of cortisol on Na(+)-K(+)-ATPase activity. As judged by several criteria, explants from pyloric ceca and the posterior region of the intestine remained viable during short-term (6-day) culture, although Na(+)-K(+)-ATPase activity declined and basolateral components of the enterocytes were observed to be partially degraded. Addition of cortisol to the culture medium maintained Na(+)-K(+)-ATPase activity (over 2-12 days) above that of control explants and, in some cases, was similar to levels before culture. The response to cortisol was dose dependent (0.001-10 microg/ml). Within the physiological range, the response was specific for cortisol and showed the following hierarchy: dexamethasone >/= cortisol > 11-deoxycortisol > cortisone. Insulin maintained Na(+)-K(+)-ATPase activity over controls in explants of ceca but not posterior intestine. To compare in vivo and in vitro responses, slow-release implants of cortisol (50 microg/g) were administered to salmon for 7 days. This treatment elevated plasma cortisol levels and stimulated Na(+)-K(+)-ATPase activity in both intestinal regions. The results demonstrate that the teleost intestine is a direct target of cortisol, this corticosteroid protects in vitro functionality of Na(+)-K(+)-ATPase, and explants retain cortisol responsiveness during short-term culture.
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Details
- Title
- Tissue culture of sockeye salmon intestine: functional response of Na+-K+-ATPase to cortisol
- Creators
- Philip A Veillette - Department of Zoology, University of Otago, Dunedin, New Zealand. pveillette@gso.uri.eduGraham Young
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.288(6), pp.R1598-1605
- Academic Unit
- UNKNOWN
- Publisher
- United States
- Identifiers
- 99900546878401842
- Language
- English
- Resource Type
- Journal article