Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate

Xueming Wei; Tingfen Yan; Norman G Hommes; Xiuduan Liu; Liyou Wu; Crystal McAlvin; Martin G Klotz; Luis A Sayavedra-Soto; Jizhong Zhou; Daniel J Arp

doi:10.1111/j.1574-6968.2006.00152.x

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Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate

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Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate

Xueming Wei, Tingfen Yan, Norman G Hommes, Xiuduan Liu, Liyou Wu, Crystal McAlvin, Martin G Klotz, Luis A Sayavedra-Soto, Jizhong Zhou and Daniel J Arp

FEMS microbiology letters, Vol.257(1), pp.76-83

04/01/2006

DOI: https://doi.org/10.1111/j.1574-6968.2006.00152.x

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https://hdl.handle.net/2376/112622

PMID: 16553835

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Abstract

starvation

ammonia deprivation

transcript profiles

nitrifier

gene expression

microarrays

Abstract The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.

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Title: Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate
Creators: Xueming Wei - Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA
Tingfen Yan - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Norman G Hommes - Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA
Xiuduan Liu - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Liyou Wu - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Crystal McAlvin - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Martin G Klotz - Department of Biology, University of Louisville, KY, USA
Luis A Sayavedra-Soto - Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA
Jizhong Zhou - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Daniel J Arp - Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA
Publication Details: FEMS microbiology letters, Vol.257(1), pp.76-83
Academic Unit: Molecular Biosciences, School of
Publisher: Blackwell Publishing Ltd; Oxford, UK
Identifiers: 99900548124601842
Language: English
Resource Type: Journal article