Transcription-coupled repair in RNA polymerase I-transcribed genes of yeast

Antonio Conconi; Vyacheslav A Bespalov; Michael J Smerdon

doi:10.1073/pnas.022373099

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Transcription-coupled repair in RNA polymerase I-transcribed genes of yeast

Journal article

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Transcription-coupled repair in RNA polymerase I-transcribed genes of yeast

Antonio Conconi, Vyacheslav A Bespalov and Michael J Smerdon

Proceedings of the National Academy of Sciences - PNAS, Vol.99(2), pp.649-654

01/22/2002

DOI: https://doi.org/10.1073/pnas.022373099

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https://hdl.handle.net/2376/116938

PMCID: PMC117360

PMID: 11782531

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Abstract

Biological Sciences

Nucleotide excision repair (NER) of UV-induced cyclobutane pyrimidine dimers (CPDs) was measured in the individual strands of transcriptionally active and inactive ribosomal genes of yeast. Ribosomal genes (rDNA) are present in multiple copies, but only a fraction of them is actively transcribed. Restriction enzyme digestion was used to specifically release the transcriptionally active fraction from yeast nuclei, and selective psoralen crosslinking was used to distinguish between active and inactive rDNA chromatin. Removal of CPDs was followed in both rDNA populations, and the data clearly show that strand-specific repair occurs in transcriptionally active rDNA while being absent in the inactive rDNA fraction. Thus, transcription-coupled repair occurs in RNA polymerase I-transcribed genes in yeast. Moreover, the nontranscribed strand of active rDNA is repaired faster than either strand of inactive rDNA, implying that NER has preferred access to the active, non-nucleosomal rDNA chromatin. Finally, restriction enzyme accessibility to active rDNA varies during NER, suggesting that there is a change in ribosomal gene chromatin structure during or soon after CPD removal.

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Title: Transcription-coupled repair in RNA polymerase I-transcribed genes of yeast
Creators: Antonio Conconi - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660
Vyacheslav A Bespalov - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660
Michael J Smerdon - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.99(2), pp.649-654
Academic Unit: Molecular Biosciences, School of
Publisher: The National Academy of Sciences
Identifiers: 99900547607501842
Language: English
Resource Type: Journal article