Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV

L.A Sayavedra-Soto; N.G Hommes; J.J Alzerreca; D.J Arp; J.M Norton; M.G Klotz

doi:10.1016/S0378-1097(98)00367-X

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Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV

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Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV

L.A Sayavedra-Soto, N.G Hommes, J.J Alzerreca, D.J Arp, J.M Norton and M.G Klotz

FEMS microbiology letters, Vol.167(1), pp.81-88

1998

DOI: https://doi.org/10.1016/S0378-1097(98)00367-X

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https://hdl.handle.net/2376/116235

PMID: 9785456

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Abstract

Nitrifier

Sequence comparison

Transcription

Ammonia monooxygenase

Nitrifying bacteria such as Nitrosomonas europaea and Nitrosospira sp. NpAV use ammonia monooxygenase (AMO) for oxidation of their primary growth substrate, ammonia. Two polypeptides of AMO are coded for by contiguous genes, amoA and amoB, which are preceded by a third gene, amoC. The amoCAB clusters are present in multiple copies in nitrifying bacteria of the β subdivision. These bacteria also have one amoC copy that is not adjacent to a copy of amoAB. The seven known amoC genes in different nitrifiers code for similar polypeptides (>68%). Reverse transcriptase-polymerase chain reactions and Northern blots indicated that amoC from the amoCAB cluster is contained on a transcript with amoAB. Two other transcripts were detected with amo probes and may be a product of processing of the amoCAB mRNA or independent transcripts.

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Title: Transcription of the amoC, amoA and amoB genes in Nitrosomonas europaea and Nitrosospira sp. NpAV
Creators: L.A Sayavedra-Soto - Laboratory for Nitrogen Fixation Research, 2082 Cordley, Oregon State University, Corvallis, OR 97331-2902, USA
N.G Hommes - Laboratory for Nitrogen Fixation Research, 2082 Cordley, Oregon State University, Corvallis, OR 97331-2902, USA
J.J Alzerreca - Department of Plants, Soil and Biometeorology, Utah State University, Logan, UT 84322-4820, USA
D.J Arp - Laboratory for Nitrogen Fixation Research, 2082 Cordley, Oregon State University, Corvallis, OR 97331-2902, USA
J.M Norton - Department of Plants, Soil and Biometeorology, Utah State University, Logan, UT 84322-4820, USA
M.G Klotz - University of Louisville, Department of Biology, Louisville, KY 40292, USA
Publication Details: FEMS microbiology letters, Vol.167(1), pp.81-88
Academic Unit: Molecular Biosciences, School of
Publisher: Elsevier B.V
Identifiers: 99900547567001842
Language: English
Resource Type: Journal article