Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana

Sven K Nelson; Camille M Steber

doi:10.1371/journal.pone.0179143

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Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana

Journal article

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Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana

Sven K Nelson and Camille M Steber

PloS one, Vol.12(6), pp.e0179143-e0179143

2017

DOI: https://doi.org/10.1371/journal.pone.0179143

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https://hdl.handle.net/2376/108359

PMCID: PMC5476249

PMID: 28628628

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Abstract

Germination - drug effects

Arabidopsis Proteins - genetics

Arabidopsis - drug effects

Arabidopsis - growth & development

Seeds - drug effects

Seeds - genetics

Alkyl and Aryl Transferases - metabolism

Receptors, Cell Surface - metabolism

Transcriptome - drug effects

RNA, Plant - metabolism

Plant Dormancy - drug effects

Arabidopsis - genetics

Arabidopsis Proteins - metabolism

Phenotype

Plants, Genetically Modified - metabolism

Seeds - growth & development

Alkyl and Aryl Transferases - genetics

Proteolysis

Gibberellins - pharmacology

Mutation

Plant Growth Regulators - pharmacology

Real-Time Polymerase Chain Reaction

Receptors, Cell Surface - genetics

While widespread transcriptome changes were previously observed with seed dormancy loss, this study specifically characterized transcriptional changes associated with the increased seed dormancy and dormancy loss of the gibberellin (GA) hormone-insensitive sleepy1-2 (sly1-2) mutant. The SLY1 gene encodes the F-box subunit of an SCF E3 ubiquitin ligase needed for GA-triggered proteolysis of DELLA repressors of seed germination. DELLA overaccumulation in sly1-2 seeds leads to increased dormancy that can be rescued without DELLA protein destruction either by overexpression of the GA receptor, GA-INSENSITIVE DWARF1b (GID1b-OE) (74% germination) or by extended dry after-ripening (11 months, 51% germination). After-ripening of sly1 resulted in different transcriptional changes in early versus late Phase II of germination that were consistent with the processes known to occur. Approximately half of the transcriptome changes with after-ripening appear to depend on SLY1-triggered DELLA proteolysis. Given that many of these SLY1/GA-dependent changes are genes involved in protein translation, it appears that GA signaling increases germination capacity in part by activating translation. While sly1-2 after-ripening was associated with transcript-level changes in 4594 genes over two imbibition timepoints, rescue of sly1-2 germination by GID1b-OE was associated with changes in only 23 genes. Thus, a big change in sly1-2 germination phenotype can occur with relatively little change in the global pattern of gene expression during the process of germination. Most GID1b-OE-responsive transcripts showed similar changes with after-ripening in early Phase II of imbibition, but opposite changes with after-ripening by late Phase II. This suggests that GID1b-OE stimulates germination early in imbibition, but may later trigger negative feedback regulation.

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Title: Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana
Creators: Sven K Nelson - Molecular Plant Sciences Program, Washington State University, Pullman, Washington, United States of America
Camille M Steber - Department of Crop and Soil Science, Washington State University, Pullman, Washington, United States of America
Publication Details: PloS one, Vol.12(6), pp.e0179143-e0179143
Academic Unit: Crop and Soil Sciences, Department of
Publisher: United States
Identifiers: 99900547479301842
Language: English
Resource Type: Journal article