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CC BY V4.0, Open Access
Journal article
Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize
Frontiers in plant science, Vol.7(2016), pp.1477-1477
10/07/2016
Handle:
https://hdl.handle.net/2376/114195
PMCID: PMC5054024
PMID: 27774095
Abstract
Originating in a tropical climate, maize has faced great challenges as cultivation has expanded to the majority of the world's temperate zones. In these zones, frost and cold temperatures are major factors that prevent maize from reaching its full yield potential. Among 30 elite maize inbred lines adapted to northern China, we identified two lines of extreme, but opposite, freezing tolerance levels—highly tolerant and highly sensitive. During the seedling stage of these two lines, we used RNA-seq to measure changes in maize whole genome transcriptome before and after freezing treatment. In total, 19,794 genes were expressed, of which 4550 exhibited differential expression due to either treatment (before or after freezing) or line type (tolerant or sensitive). Of the 4550 differently expressed genes, 948 exhibited differential expression due to treatment within line or lines under freezing condition. Analysis of gene ontology found that these 948 genes were significantly enriched for binding functions (DNA binding, ATP binding, and metal ion binding), protein kinase activity, and peptidase activity. Based on their enrichment, literature support, and significant levels of differential expression, 30 of these 948 genes were selected for quantitative real-time PCR (qRT-PCR) validation. The validation confirmed our RNA-Seq-based findings, with squared correlation coefficients of 80% and 50% in the tolerance and sensitive lines, respectively. This study provided valuable resources for further studies to enhance understanding of the molecular mechanisms underlying maize early freezing response and enable targeted breeding strategies for developing varieties with superior frost resistance to achieve yield potential.
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Details
- Title
- Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize
- Creators
- Zhao Li - Agronomy College of Northeast Agricultural UniversityGuanghui Hu - Agronomy College of Northeast Agricultural UniversityXiangfeng Liu - Agronomy College of Northeast Agricultural UniversityYao Zhou - Agronomy College of Northeast Agricultural UniversityYu Li - Institute of Crop Science, Chinese Academy of Agricultural SciencesXu Zhang - Department of Crop and Soil Sciences, Washington State UniversityXiaohui Yuan - Department of Crop and Soil Sciences, Washington State UniversityQian Zhang - Agronomy College of Northeast Agricultural UniversityDeguang Yang - Agronomy College of Northeast Agricultural UniversityTianyu Wang - Institute of Crop Science, Chinese Academy of Agricultural SciencesZhiwu Zhang - Agronomy College of Northeast Agricultural University
- Publication Details
- Frontiers in plant science, Vol.7(2016), pp.1477-1477
- Academic Unit
- Department of Crop and Soil Sciences
- Publisher
- Frontiers Media S.A
- Grant note
- 2015M571383 / China Postdoctoral Science Foundation
- Identifiers
- 99900547427201842
- Language
- English
- Resource Type
- Journal article