Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung

S T Kanaly; S A Hines; G H Palmer

doi:10.1128/IAI.64.4.1126-1132.1996

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Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung

Journal article

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Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung

S T Kanaly, S A Hines and G H Palmer

Infection and immunity, Vol.64(4), pp.1126-1132

04/1996

DOI: https://doi.org/10.1128/IAI.64.4.1126-1132.1996

Handle:

https://hdl.handle.net/2376/115160

PMCID: PMC173893

PMID: 8606068

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https://doi.org/10.1128/IAI.64.4.1126-1132.1996View

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Abstract

Cell Line

Lung - microbiology

Lung - pathology

Immunotherapy, Adoptive

Cytokines - analysis

RNA, Messenger - analysis

Th1 Cells - immunology

Rhodococcus equi - immunology

Animals

Mice, Nude

Female

Mice

Mice, Inbred BALB C

Cytokines - genetics

Lung - immunology

Rhodococcus equi, and intracellular respiratory pathogen, causes sever e granulomatous pneumonia in humans with AIDS and in young horses. Pulmonary clearance of R. equi requires functional CD4+ T cells and gamma interferon (IFN-gamma) expression from bronchial lymph node cells. The purpose of this study was to investigate whether R. equi-specific CD4+ Th1 cells could effect clearance of R. equi from the lung. Adoptive transfer of a clearance of R. equi from the lungs. In contrast, mice transfused with a R. equi-specific CD4+ Th2 cell line expressed interleukin-4 but not IFN-gamma mRNA, failed to clear pulmonary infection, and developed granulomas in the lung. Control mice, which did not receive cells, did not produce IFN-gamma or interleukin-4 and developed small pulmonary granulomas. These results clearly show that a Th1 response is sufficient to effect pulmonary clearance of R. equi.

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Title: Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung
Creators: S T Kanaly - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
S A Hines
G H Palmer
Publication Details: Infection and immunity, Vol.64(4), pp.1126-1132
Academic Unit: Veterinary Medicine, College of; Paul G. Allen School for Global Animal Health
Publisher: United States
Grant note: AI 01233-01 / NIAID NIH HHS
Identifiers: 99900547838001842
Language: English
Resource Type: Journal article