Transferrin gene expression and synthesis by cultured choroid plexus epithelial cells: Regulation by serotonin and cyclic adenosine 3',5'-monophosphate

M Tsutsumi; M K Skinner; E Sanders-Bush

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Transferrin gene expression and synthesis by cultured choroid plexus epithelial cells: Regulation by serotonin and cyclic adenosine 3',5'-monophosphate

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Transferrin gene expression and synthesis by cultured choroid plexus epithelial cells: Regulation by serotonin and cyclic adenosine 3',5'-monophosphate

M Tsutsumi, M K Skinner and E Sanders-Bush

The Journal of biological chemistry, Vol.264(16), pp.9626-9631

06/05/1989

Handle:

https://hdl.handle.net/2376/101068

PMID: 2542315

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Abstract

Epithelium - metabolism

RNA, Messenger - isolation & purification

Cells, Cultured

Choroid Plexus - metabolism

Epithelial Cells

Transferrin - physiology

Rats, Inbred Strains

Serotonin - pharmacology

Transferrin - genetics

Cyclic AMP - pharmacology

Gene Expression Regulation - drug effects

Blotting, Northern

Choroid Plexus - cytology

Transferrin - biosynthesis

Genetics

Primary cultures of rat choroid plexus epithelial cells were established and used to investigate the role of the choroid plexus in the synthesis and secretion of transferrin. Transferrin gene expression was determined by a Northern blot analysis with a transferrin cRNA probe. A single transferrin mRNA species was detected and found to be the same size as the transcripts in the liver and Sertoli cells. Immunoprecipitation of radiolabeled secreted proteins with an antiserum transferrin antibody demonstrated that cultured choroid plexus epithelial cells synthesize and secrete a 70-kDa species of transferrin. Levels of transferrin secretion by rat choroid plexus epithelial cells in culture were measured by radioimmunoassay. Treatment of the choroid plexus epithelial cells in culture with cell-permeable cAMP analogs or serotonin led to time- and concentration-dependent changes in the levels of transferrin in the medium. Dibutyryl-cAMP and 8-bromo-cAMP decreased the levels of transferrin synthesized and secreted by choroid plexus epithelial cells with an EC50 value of 30 nM. Serotonin, however, increased the levels of transferrin with an EC50 value of 100 nM. A concomitant change in transferrin mRNA concentrations was observed in response to serotonin. These data suggest that the synthesis of transferrin by the choroid plexus is reciprocally regulated by the neurotransmitter serotonin and by regulatory agents coupled to adenylate cyclase. Regulatory agents such as serotonin may have a critical role in modulating the proteins synthesized by the choroid plexus, thereby influencing the composition of the cerebrospinal fluid.

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Title: Transferrin gene expression and synthesis by cultured choroid plexus epithelial cells
Creators: M Tsutsumi - Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
M K Skinner
E Sanders-Bush
Publication Details: The Journal of biological chemistry, Vol.264(16), pp.9626-9631
Academic Unit: Biological Sciences, School of
Publisher: United States
Grant note: MH34007 / NIMH NIH HHS HD20583 / NICHD NIH HHS
Identifiers: 99900546556301842
Language: English
Resource Type: Journal article