Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice

Timothy J Doyle; Jennifer L Bowman; Veronica L Windell; Derek J McLean; Kwan Hee Kim

doi:10.1095/biolreprod.112.106104

Back

Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice

Journal article

Open access

Peer reviewed

Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice

Timothy J Doyle, Jennifer L Bowman, Veronica L Windell, Derek J McLean and Kwan Hee Kim

Biology of reproduction, Vol.88(5), pp.112-112

05/2013

DOI: https://doi.org/10.1095/biolreprod.112.106104

Handle:

https://hdl.handle.net/2376/110486

PMID: 23536373

Files and links (1)

url

https://doi.org/10.1095/biolreprod.112.106104View

Published (Version of record) Open

Abstract

Spermatogonia - drug effects

Sperm Count

Spermatogenesis - drug effects

Prenatal Exposure Delayed Effects

Testis - cytology

Apoptosis - drug effects

Male

Stem Cells - cytology

Plasticizers - toxicity

Spermatozoa - drug effects

Testis - drug effects

Pregnancy

Sperm Motility - drug effects

Animals

Diethylhexyl Phthalate - toxicity

Stem Cells - drug effects

Female

Mice

Spermatozoa - cytology

Spermatogonia - cytology

Seminiferous Tubules - drug effects

Recent evidence has linked human phthalate exposure to abnormal reproductive and hormonal effects. Phthalates are plasticizers that confer flexibility and transparency to plastics, but they readily contaminate the body and the environment. In this study, timed pregnant CD1 outbred mice were treated with di-(2-ethylhexyl) phthalate (DEHP) from Embryonic Day 7 (E7) to E14. The subsequent generation (F1) offspring were then bred to produce the F2, F3, and F4 offspring, without any further DEHP treatment. This exposure scheme disrupted testicular germ cell association and decreased sperm count and motility in F1 to F4 offspring. By spermatogonial transplantation techniques, the exposure scheme also disrupted spermatogonial stem cell (SSC) function of F3 offspring. The W/W(V) recipient testes transplanted with F3 offspring germ cells from the DEHP-treated group had a dramatically lower percentage of donor germ cell-derived spermatogenic recovery in seminiferous tubules when compared to the recipient testes transplanted with CD1 control germ cells. Further characterization showed that the major block of donor germ cell-derived spermatogenesis was before the appearance of undifferentiated spermatogonia. Interestingly, the testes transplanted with the F3 offspring germ cells from the DEHP-treated group, when regenerated, replicated testis morphology similar to that observed in the testes from the F1 to F3 offspring of the DEHP-treated group, suggesting that the germ cell disorganization phenotype originates from the stem cells of F3 offspring. In conclusion, embryonic exposure to DEHP was found to disrupt testicular germ cell organization and SSC function in a transgenerational manner.

Metrics

20 Record Views

See more details

Details

Title: Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice
Creators: Timothy J Doyle - School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
Jennifer L Bowman
Veronica L Windell
Derek J McLean
Kwan Hee Kim
Publication Details: Biology of reproduction, Vol.88(5), pp.112-112
Academic Unit: Center for Reproductive Biology
Publisher: United States
Grant note: ES019836 / NIEHS NIH HHS T32 GM008336 / NIGMS NIH HHS R01 ES019836 / NIEHS NIH HHS
Identifiers: 99900547376601842
Language: English
Resource Type: Journal article