Transgenerational epigenetic programming of the embryonic testis transcriptome

Matthew D ANWAY; Stephen S REKOW; Michael K SKINNER

doi:10.1016/j.ygeno.2007.10.002

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Transgenerational epigenetic programming of the embryonic testis transcriptome

Journal article

Open access

Peer reviewed

Transgenerational epigenetic programming of the embryonic testis transcriptome

Matthew D ANWAY, Stephen S REKOW and Michael K SKINNER

Genomics (San Diego, Calif.), Vol.91(1), pp.30-40

2008

DOI: https://doi.org/10.1016/j.ygeno.2007.10.002

Handle:

https://hdl.handle.net/2376/105573

PMCID: PMC2239263

PMID: 18042343

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Abstract

Fundamental and applied biological sciences. Psychology

Cell differentiation, maturation, development, hematopoiesis

Genes. Genome

Biological and medical sciences

Molecular and cellular biology

Genetics of eukaryotes. Biological and molecular evolution

Molecular Genetics

Cell Physiology

Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination appears to promote an epigenetic reprogramming of the male germ line that is associated with transgenerational adult-onset disease states. Transgenerational effects on the embryonic day 16 (E16) testis demonstrated reproducible changes in the testis transcriptome for multiple generations (F1-F3). The expression of 196 genes was found to be influenced, with the majority of gene expression being decreased or silenced. Dramatic changes in the gene expression of methyltransferases during gonadal sex determination were observed in the F1 and F2 vinclozolin generation (E16) embryonic testis, but the majority returned to control-generation levels by the F3 generation. The most dramatic effects were on the germ-line-associated Dnmt3A and Dnmt3L isoforms. Observations demonstrate that an embryonic exposure to vinclozolin appears to promote an epigenetic reprogramming of the male germ line that correlates with transgenerational alterations in the testis transcriptome in subsequent generations. Published by Elsevier Inc.

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Details

Title: Transgenerational epigenetic programming of the embryonic testis transcriptome
Creators: Matthew D ANWAY - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4231, United States
Stephen S REKOW - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4231, United States
Michael K SKINNER - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4231, United States
Publication Details: Genomics (San Diego, Calif.), Vol.91(1), pp.30-40
Academic Unit: Biological Sciences, School of
Publisher: Elsevier; San Diego, CA
Number of pages: 11
Grant note: R01 ES012974-03; R01 ES012974-03S1; R01 ES012974 / NIEHS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) R01ES012974 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
Identifiers: 99900546940401842
Language: English
Resource Type: Journal article