Journal article
Transgenic miR132 Alters Neuronal Spine Density and Impairs Novel Object Recognition Memory
PloS one, Vol.5(11), pp.e15497-e15497
2010
Handle:
https://hdl.handle.net/2376/109661
PMCID: PMC2993964
PMID: 21124738
Abstract
Inducible gene expression plays a central role in neuronal plasticity, learning, and memory, and dysfunction of the underlying molecular events can lead to severe neuronal disorders. In addition to coding transcripts (mRNAs), non-coding microRNAs (miRNAs) appear to play a role in these processes. For instance, the CREB-regulated miRNA miR132 has been shown to affect neuronal structure in an activity-dependent manner, yet the details of its physiological effects and the behavioral consequences
in vivo
remain unclear. To examine these questions, we employed a transgenic mouse strain that expresses miR132 in forebrain neurons. Morphometric analysis of hippocampal neurons revealed that transgenic miR132 triggers a marked increase in dendritic spine density. Additionally, miR132 transgenic mice exhibited a decrease in the expression of MeCP2, a protein implicated in Rett Syndrome and other disorders of mental retardation. Consistent with these findings, miR132 transgenic mice displayed significant deficits in novel object recognition. Together, these data support a role for miR132 as a regulator of neuronal structure and function, and raise the possibility that dysregulation of miR132 could contribute to an array of cognitive disorders.
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Details
- Title
- Transgenic miR132 Alters Neuronal Spine Density and Impairs Novel Object Recognition Memory
- Creators
- Katelin F HansenKensuke SakamotoGary A WaymanSoren ImpeyKarl Obrietan
- Publication Details
- PloS one, Vol.5(11), pp.e15497-e15497
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Public Library of Science; San Francisco, USA
- Identifiers
- 99900547218901842
- Language
- English
- Resource Type
- Journal article