Journal article
Transposon Mutagenesis of Salmonella enterica Serovar Enteritidis Identifies Genes That Contribute to Invasiveness in Human and Chicken Cells and Survival in Egg Albumen
Infection and immunity, Vol.80(12), pp.4203-4215
12/2012
Handle:
https://hdl.handle.net/2376/116797
PMCID: PMC3497420
PMID: 22988017
Abstract
Salmonella enterica
serovar Enteritidis is an important food-borne pathogen, and chickens are a primary reservoir of human infection. While most knowledge about
Salmonella
pathogenesis is based on research conducted on
Salmonella enterica
serovar Typhimurium,
S
. Enteritidis is known to have pathobiology specific to chickens that impacts epidemiology in humans. Therefore, more information is needed about
S
. Enteritidis pathobiology in comparison to that of
S
. Typhimurium. We used transposon mutagenesis to identify
S
. Enteritidis virulence genes by assay of invasiveness in human intestinal epithelial (Caco-2) cells and chicken liver (LMH) cells and survival within chicken (HD-11) macrophages as a surrogate marker for virulence. A total of 4,330 transposon insertion mutants of an invasive G1 Nal
r
strain were screened using Caco-2 cells. This led to the identification of attenuating mutations in a total of 33 different loci, many of which include genes previously known to contribute to enteric infection (e.g.,
Salmonella
pathogenicity island 1 [SPI-1], SPI-4, SPI-5, CS54,
fliH
,
fljB
,
csgB
,
spvR
, and
rfbMN
) in
S
. Enteritidis and other
Salmonella
serovars. Several genes or genomic islands that have not been reported previously (e.g., SPI-14,
ksgA
,
SEN0034
,
SEN2278
, and
SEN3503
) or that are absent in
S
. Typhimurium or in most other
Salmonella
serovars (e.g.,
pegD
,
SEN1152
,
SEN1393
, and
SEN1966
) were also identified. Most mutants with reduced Caco-2 cell invasiveness also showed significantly reduced invasiveness in chicken liver cells and impaired survival in chicken macrophages and in egg albumen. Consequently, these genes may play an important role during infection of the chicken host and also contribute to successful egg contamination by
S
. Enteritidis.
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Details
- Title
- Transposon Mutagenesis of Salmonella enterica Serovar Enteritidis Identifies Genes That Contribute to Invasiveness in Human and Chicken Cells and Survival in Egg Albumen
- Creators
- Devendra H Shah - Department of Veterinary Microbiology and PathologyXiaohui Zhou - Department of Veterinary Microbiology and PathologyHye-Young Kim - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USADouglas R Call - Department of Veterinary Microbiology and PathologyJean Guard - Egg Quality and Safety Research Unit, Agriculture Research Service, United States Department of Agriculture, Athens, Georgia, USA
- Publication Details
- Infection and immunity, Vol.80(12), pp.4203-4215
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547436101842
- Language
- English
- Resource Type
- Journal article