Journal article
Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses
Veterinary immunology and immunopathology, Vol.140(3-4), pp.244-251
04/15/2011
Handle:
https://hdl.handle.net/2376/107673
PMID: 21288576
Abstract
Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization site by administration of bovine Flt3L and GM-CSF prior to inoculation with DNA vaccine constructs and MVA boost was evaluated. Analysis of immune responses showed induction of significant T. parva-specific proliferation, and IFN-γ-secreting CD4+ and CD8+ T cell responses in immunized cattle. However, antigen-specific CTLs were not detected. Following lethal challenge, 5/12 immunized cattle survived by day 21, whereas all the negative controls had to be euthanized due to severe disease, indicating a protective effect of the vaccine (p<0.05). The study demonstrated the potential of this technology to elicit significant MHC class II and class I restricted IFN-γ-secreting CD4+ and CD8+ T cells to defined vaccine candidate antigens in a natural host, but also underscores the need to improve strategies for eliciting protective CTL responses.
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Details
- Title
- Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses
- Creators
- Duncan M Mwangi - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaYoshikazu Honda - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaSimon P Graham - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaRoger Pelle - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaEvans L.N Taracha - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaJames Gachanja - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaJohn K Nyanjui - International Livestock Research Institute, P. O. Box 30709, Nairobi 00100, KenyaJocelyn Bray - Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4467, USAGuy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAWendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAWaithaka Mwangi - Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4467, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.140(3-4), pp.244-251
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900546872601842
- Language
- English
- Resource Type
- Journal article