Treponema pallidum receptor binding proteins interact with fibronectin

K M Peterson; J B Baseman; J F Alderete

doi:10.1084/jem.157.6.1958

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Treponema pallidum receptor binding proteins interact with fibronectin

Journal article

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Treponema pallidum receptor binding proteins interact with fibronectin

K M Peterson, J B Baseman and J F Alderete

The Journal of experimental medicine, Vol.157(6), pp.1958-1970

06/01/1983

DOI: https://doi.org/10.1084/jem.157.6.1958

Handle:

https://hdl.handle.net/2376/112957

PMID: 6304227

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Abstract

Cell Line

Immune Sera - pharmacology

Fibronectins - immunology

Iodine Radioisotopes

Treponema pallidum - metabolism

Humans

Glass

Receptors, Cell Surface - metabolism

Blood Proteins - metabolism

Fibronectins - metabolism

Protein Binding - drug effects

Bacterial Proteins - metabolism

Receptors, Fibronectin

Analysis of plasma proteins avidly bound to T. pallidum surfaces revealed the ability of T. pallidum to acquire numerous host macromolecules. No acquisition was evident by the avirulent spirochete, T. phagedenis biotype Reiter. Western blotting technology using hyperimmune antifibronectin serum as a probe revealed the ability of virulent treponemes to avidly bind fibronectin from a complex medium such as plasma. The specificity of the tiplike adherence of motile T. pallidum to fibronectin-coated glass surfaces and to fibronectin on HEp-2 cells was reinforced by the observation that pretreatment of coverslips or cell monolayers with monospecific antiserum against fibronectin substantially reduced T. pallidum attachment. The stoichiometric binding of T. pallidum to fibronectin-coated coverslips and the inability of unlabeled or 35S-radiolabeled treponemes to interact with glass surfaces treated with other plasma proteins further established the specific nature of the interaction between virulent T. pallidum and fibronectin. The avid association between three outer envelope proteins of T. pallidum and fibronectin was also demonstrated. These treponemal surface proteins have been previously identified as putative receptor-binding proteins responsible for T. pallidum parasitism of host cells. The data suggest that surface fibronectin mediates tip-oriented attachment of T. pallidum to host cells via a receptor-ligand mechanism of recognition.

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Title: Treponema pallidum receptor binding proteins interact with fibronectin
Creators: K M Peterson
J B Baseman
J F Alderete
Publication Details: The Journal of experimental medicine, Vol.157(6), pp.1958-1970
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: AI-15036 / NIAID NIH HHS 1-K04-A1-00178 / PHS HHS
Identifiers: 99900547548001842
Language: English
Resource Type: Journal article