Journal article
Tricalcium phosphate and tricalcium phosphate/polycaprolactone particulate composite for controlled release of protein
Materials Science & Engineering C, Vol.33(7), pp.3576-3582
10/2013
Handle:
https://hdl.handle.net/2376/109080
PMCID: PMC3734380
PMID: 23910252
Abstract
β-Tricalcium phosphate (β-TCP) with three different particle size ranges was used to study the effects of particle size and surface area on protein adsorption and release. Polycaprolactone (PCL) coating was applied on the particle systems to investigate its effect on particulate system properties from both structural and application aspects. The maximum loading of 27mg/g was achieved for 100nm particles. Bovine serum albumin (BSA) loading amount was controlled by varying the BSA loading solution concentration, as well as the sample powder's surface area. Increasing the surface area of the delivery powder significantly increased loading and release yield. Unlike the samples with low surface area, the lowest particle size samples showed sigmoidal release profile. This indicated that release was governed by different mechanisms for particles with different sizes. While the majority of samples showed no more than 50% release, the 550nm particles demonstrated 100% release. PCL coating showed no significant ability to attenuate burst release in PBS. However, it led to a steadier release profile as compared to the bare TCP particles. FTIR analysis also proved that the secondary structure of BSA did not change significantly during the adsorption; however, minor denaturation was found during the release. The same results were found when PCL coating was applied on the TCP particles. We envision potential use of TCP and TCP+PCL systems in bone growth factor or orthopedic drug delivery applications in future bone tissue engineering application.
•BSA adsorption and release kinetics is dependent on particle size and initial protein concentration.•BSA release mechanism is governed by particle size.•PCL coating cannot retard burst release effect.•Insignificant protein denaturation occurs using TCP/TCP+PCL systems.
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Details
- Title
- Tricalcium phosphate and tricalcium phosphate/polycaprolactone particulate composite for controlled release of protein
- Creators
- Sahar VahabzadehJoe EdgingtonSusmita Bose
- Publication Details
- Materials Science & Engineering C, Vol.33(7), pp.3576-3582
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547289401842
- Language
- English
- Resource Type
- Journal article