Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression

D P Jasmer

doi:10.1083/jcb.121.4.785

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Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression

Journal article

Open access

Peer reviewed

Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression

D P Jasmer

The Journal of cell biology, Vol.121(4), pp.785-793

05/1993

DOI: https://doi.org/10.1083/jcb.121.4.785

Handle:

https://hdl.handle.net/2376/105232

PMCID: PMC2119794

PMID: 8491772

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https://doi.org/10.1083/jcb.121.4.785View

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Abstract

Gene Expression

Muscles - parasitology

Molecular Sequence Data

Trichinellosis - pathology

Blotting, Northern

Animals

Cell Cycle

Trichinellosis - genetics

Base Sequence

DNA - biosynthesis

Transcription, Genetic

Trichinella spiralis - physiology

Mice

Muscles - metabolism

Muscles - cytology

Infection by Trichinella spiralis causes a variety of changes in skeletal muscle cells including the hypertrophy of nuclei and decreased expression of muscle specific proteins. Potential cellular processes leading to these changes were investigated. In synchronized muscle infections, [3H]thymidine was incorporated into infected cell nuclei from 2-5 days post infection. Labeled nuclei were stably integrated into the infected cell up to 60 days post infection and appear to originate from differentiated skeletal muscle nuclei present at the time of infection. These nuclei were further shown to contain a mean DNA content of approximately 4N, indicating that the [3H]thymidine uptake reflects DNA synthesis and subsequent long-term suspension of the infected cell in the cell cycle at G2/M. Associated with these changes, muscle specific gene transcripts were reduced to < 1- < 0.1% in the infected cell compared to normal muscle. Transcript levels of the muscle transcriptional regulatory factors myogenin, MyoD1, and Id were reduced to < 10, < 1, and increased approximately 250%, respectively, in the infected cell compared to normal muscle, indicating transcriptional inactivation of muscle genes. DNA synthesis in the infected cell may represent the initiation event which leads to expression of this infected cell phenotype.

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Title: Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression
Creators: D P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040
Publication Details: The Journal of cell biology, Vol.121(4), pp.785-793
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: R29 AI 26157 / NIAID NIH HHS
Identifiers: 99900546902501842
Language: English
Resource Type: Journal article