Journal article
Trichomonas vaginalis: Electrophoretic analysis and heterogeneity among isolates due to high-molecular-weight trichomonad proteins
Experimental parasitology, Vol.61(2), pp.244-251
1986
Handle:
https://hdl.handle.net/2376/113481
PMID: 3485537
Abstract
The protein composition of
Trichomonas vaginalis isolates was evaluated using one-dimensional and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. At least two hundred intrinsically labeled and about 30 major extrinsically labeled proteins of molecular weights less than 120,000 were resolved by isoelectric focusing and electrophoresis. In general, long-term grown and fresh isolates of
T. vaginalis gave similar fluorograms of the readily detectable proteins. Only a minor variance in a distinct protein was noted among three of the four fresh isolates tested. Labeling with [
35S]methionine or
3H-amino acids gave almost identical profiles, ensuring the efficient radiolabeling of trichomonad proteins that dominate quantitatively. Comparative analysis of radioactivity profiles of one-dimensional gels emphasizing the region of high-molecular-weight proteins known to reside in low copy number revealed the presence or absence of the internally synthesized proteins from surfaces of
T. vaginalis isolates. Finally, immunoblotting of two-dimensional gels demonstrated the highly immunogenic nature of proteins corresponding to quantitatively dominant molecular weight regions of intrinsically labeled proteins.
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Details
- Title
- Trichomonas vaginalis: Electrophoretic analysis and heterogeneity among isolates due to high-molecular-weight trichomonad proteins
- Creators
- John F Alderete - Department of Microbiology, The University of Texas Health Science Center; San Antonio, Texas 78284, U.S.AGuillermo Garza - Department of Microbiology, The University of Texas Health Science Center; San Antonio, Texas 78284, U.S.AJoan Smith - Department of Obstetrics and Gynecology, Hahneman Medical School, Philadelphia, Pennsylvania 19102, U.S.AMichael Spence - Department of Obstetrics and Gynecology, Hahneman Medical School, Philadelphia, Pennsylvania 19102, U.S.A
- Publication Details
- Experimental parasitology, Vol.61(2), pp.244-251
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547844401842
- Language
- English
- Resource Type
- Journal article