Journal article
Trichomonas vaginalis Has Two Fibronectin-Like Iron-Regulated Genes
Archives of medical research, Vol.32(2), pp.102-107
2001
Handle:
https://hdl.handle.net/2376/113719
PMID: 11343805
Abstract
Trichomonas vaginalis, a protozoan parasite of the human urogenital tract, interacts with fibronectin (FN), a glycoprotein of the extracellular matrix. We, therefore, attempted to identify genes of this eukaryote encoding FN-binding proteins.
A cDNA clone, C1, representing an incomplete gene was obtained from an expression library based on its FN-binding ability and was characterized. The full-length 378-bp gene encoding a 14.8-kDa protein of 125 amino acids was obtained.
The amino acid sequences revealed homology with the type III-14 repeat of the heparin-binding domain at the carboxyl terminal end of FN. This fibronectin-like protein gene,
flp1, was single copy in all the
T. vaginalis isolates examined. Levels of
flp1 transcript were elevated in cells grown under low-iron conditions. Another low-iron-regulated gene,
flp2, with 70 and 67.5% identity to
flp1 at the nucleotide and amino acid levels, respectively, was recovered from the trichomonad genome. Both
flp1 and
flp2 had consensus Inr promoter-like elements immediately adjacent to the start codon.
flp2 also contained an additional Inr element followed by an ATG 24-bp within the gene.
Unlike
flp2, the
flp1 gene had AU-rich destabilizing elements in the 3′-untranslated region (UTR).
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Details
- Title
- Trichomonas vaginalis Has Two Fibronectin-Like Iron-Regulated Genes
- Creators
- Marie-Laure V CrouchJohn F Alderete
- Publication Details
- Archives of medical research, Vol.32(2), pp.102-107
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900548229101842
- Language
- English
- Resource Type
- Journal article