Journal article
Trichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic
Experimental parasitology, Vol.103(1), pp.44-50
2003
Handle:
https://hdl.handle.net/2376/101050
PMID: 12810045
Abstract
Some isolates of
Trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-RNA virus. Immune rabbit anti-capsid serum (IRS) reacted with the capsid protein of purified dsRNA virus of a subset of the virus-infected
T. vaginalis isolates. A monoclonal antibody (mAb) that recognized the capsid protein reactive with the IRS was generated. Analysis of the virus capsid protein of virus-infected isolates by probing nitrocellulose blots with mAb revealed diversity among immunoreactivity and in the size of the reactive capsid protein. Despite difficulties in visualizing virus within parasites by cross-section electron microscopy, gold-conjugated mAb readily labeled the cytoplasm of virus-positive trichomonads. Finally and importantly, isolates infecting patients attending an STD clinic, 75% of which were virus-positive isolates, had capsid protein of the same size detected by mAb present in all dsRNA viruses.
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Details
- Title
- Trichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic
- Creators
- J.F Alderete - Department of Microbiology, University of Texas Health Science Center, MC7758, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USAK.A Wendel - Division of Infectious Diseases, Johns Hopkins University School of Medicine Baltimore, MD, USAA.M Rompalo - Division of Infectious Diseases, Johns Hopkins University School of Medicine Baltimore, MD, USAE.J Erbelding - Division of Infectious Diseases, Johns Hopkins University School of Medicine Baltimore, MD, USAM Benchimol - RJ Universidade Santa Ursula, Rio de Janeiro, BrazilT.-H Chang - Department of Microbiology, University of Texas Health Science Center, MC7758, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA
- Publication Details
- Experimental parasitology, Vol.103(1), pp.44-50
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546571701842
- Language
- English
- Resource Type
- Journal article