Trichomonas vaginalis perturbs the junctional complex in epithelial cells

Rodrigo Furtado Madeiro da Costa; Wanderley de Souza; Marlene Benchimol; John F Alderete; Jose Andres Morgado-Diaz

doi:10.1038/sj.cr.7290340

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Trichomonas vaginalis perturbs the junctional complex in epithelial cells

Journal article

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Trichomonas vaginalis perturbs the junctional complex in epithelial cells

Rodrigo Furtado Madeiro da Costa, Wanderley de Souza, Marlene Benchimol, John F Alderete and Jose Andres Morgado-Diaz

Cell research, Vol.15(9), pp.704-716

09/2005

DOI: https://doi.org/10.1038/sj.cr.7290340

Handle:

https://hdl.handle.net/2376/105103

PMID: 16212877

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Abstract

Gap Junctions - metabolism

Trichomonas vaginalis - metabolism

Epithelial Cells - metabolism

Cadherins - metabolism

Humans

Actins - metabolism

Electrophysiology

Immunoblotting

Parasites

Phosphoproteins - metabolism

Occludin

Epithelial Cells - parasitology

Time Factors

Cell Membrane - metabolism

Membrane Proteins - metabolism

Caco-2 Cells

Tight Junctions - metabolism

Microscopy, Electron, Transmission

Microscopy, Electron, Scanning

Cell Survival

Electric Impedance

Cell Adhesion

Iron - metabolism

Animals

Zonula Occludens-1 Protein

Subcellular Fractions

Microscopy, Fluorescence

Trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis, which in recent years have been associated with the cervical cancer development. In the present study we analyzed the modifications at the junctional complex level of Caco-2 cells after interaction with two isolates of T. vaginalis and the influence of the iron concentration present in the parasite's culture medium on the interaction effects. Our results show that T. vaginalis adheres to the epithelial cell causing alterations in the junctional complex, such as: (a) a decrease in transepithelial electrical resistance; (b) alteration in the pattern of junctional complex proteins distribution as observed for E-cadherin, occludin and ZO-1; and (c) enlargement of the spaces between epithelial cells. These effects were dependent on (a) the degree of the parasite virulence isolate, (b) the iron concentration in the culture medium, and (c) the expression of adhesin proteins on the parasite surface.

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Title: Trichomonas vaginalis perturbs the junctional complex in epithelial cells
Creators: Rodrigo Furtado Madeiro da Costa - Programa de Pós Graduação em Ciências Morfológicas, Universidade Federal do Rio de Janeiro, Brazil
Wanderley de Souza
Marlene Benchimol
John F Alderete
Jose Andres Morgado-Diaz
Publication Details: Cell research, Vol.15(9), pp.704-716
Academic Unit: Molecular Biosciences, School of
Publisher: England
Identifiers: 99900546936301842
Language: English
Resource Type: Journal article