Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expression

J. H KANG; H. O SONG; J. S RYU; M. H SHIN; J. M KIM; Y. S CHO; J. F ALDERETE; M. H AHN; D. Y MIN

doi:10.1111/j.1365-3024.2006.00884.x

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Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expression

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Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expression

J. H KANG, H. O SONG, J. S RYU, M. H SHIN, J. M KIM, Y. S CHO, J. F ALDERETE, M. H AHN and D. Y MIN

Parasite immunology, Vol.28(9), pp.439-446

09/2006

DOI: https://doi.org/10.1111/j.1365-3024.2006.00884.x

Handle:

https://hdl.handle.net/2376/116470

PMCID: PMC2562650

PMID: 16916367

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https://doi.org/10.1111/j.1365-3024.2006.00884.xView

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Abstract

Trichomonas vaginalis

Mcl-1

apoptosis

neutrophil

caspase-3

Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with Trichomonas vaginalis infection. However, it is not known whether neutrophil apoptosis is induced by live T. vaginalis . Therefore, we examined whether T. vaginalis can influence neutrophil apoptosis, and also whether caspase-3 and the Bcl-2 family members are involved in the apoptosis. Thus, human neutrophils were incubated with live T. vaginalis and neutrophil apoptosis was evaluated by Giemsa, annexin V-PI, and DiOC 6 stainings. The neutrophil apoptosis was significantly higher in those incubated with T. vaginalis than in the control group. When trichomonads were pre-treated with mAb to AP65 (adhesin protein), or when trophozoites were separated from neutrophils using a Transwell chamber, neutrophil apoptosis was significantly reduced. The activation of caspase-3 was evident in neutrophils undergoing spontaneous apoptosis but was markedly enhanced during T. vaginalis -induced apoptosis. Moreover, the inhibition of caspase-3 effectively reduced T. vaginalis -induced apoptosis. Trichomonad-induced apoptosis was also associated with reduced expression of the neutrophil anti-apoptotic protein, Mcl-1. These results indicate that T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils.

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Title: Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expression
Creators: J. H KANG - Department of Parasitology, Hanyang University College of Medicine, Seoul, Korea
H. O SONG - Department of Parasitology, Hanyang University College of Medicine, Seoul, Korea
J. S RYU - Department of Parasitology, Hanyang University College of Medicine, Seoul, Korea
M. H SHIN - Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea
J. M KIM - Department of Microbiology, Hanyang University College of Medicine, Seoul, Korea
Y. S CHO - Shin Women’s Hospital, Uijeongbu, Korea
J. F ALDERETE - Department of Microbiology and Immunology, University of Texas Health Science Centre at San Antonio, TX, USA
M. H AHN - Department of Parasitology, Hanyang University College of Medicine, Seoul, Korea
D. Y MIN - Department of Parasitology, Hanyang University College of Medicine, Seoul, Korea
Publication Details: Parasite immunology, Vol.28(9), pp.439-446
Academic Unit: Molecular Biosciences, School of
Identifiers: 99900548108801842
Language: English
Resource Type: Journal article