Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance

Jinpeng Gao; James G Wallis; Jeremy B Jewell; John Browse

doi:10.1104/pp.17.00340

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Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance

Journal article

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Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance

Jinpeng Gao, James G Wallis, Jeremy B Jewell and John Browse

Plant physiology (Bethesda), Vol.174(3), pp.1713-1727

07/2017

DOI: https://doi.org/10.1104/pp.17.00340

Handle:

https://hdl.handle.net/2376/117934

PMID: 28495891

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Abstract

Adaptation, Physiological

Recombinant Proteins - metabolism

Amino Acid Sequence

Methyltransferases - chemistry

Arabidopsis - physiology

Arabidopsis Proteins - genetics

Cold Temperature

Arabidopsis - enzymology

Arabidopsis - growth & development

Methyltransferases - metabolism

Methyltransferases - genetics

Genetic Complementation Test

Arabidopsis - genetics

Arabidopsis Proteins - metabolism

Saccharomyces cerevisiae - metabolism

Sequence Homology, Amino Acid

Arabidopsis Proteins - chemistry

Plants, Genetically Modified

Gene Expression Regulation, Plant

Phenotype

Reproduction

Mutation

Chilling stress is a major factor limiting plant development and crop productivity. Because the plant response to chilling is so complex, we are far from understanding the genes important in the response to chilling. To identify new genes important in chilling tolerance, we conducted a novel mutant screen, combining a confirmed SALK T-DNA insertion collection with traditional forward genetics. We screened a pool of more than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonged growth at 5°C. Of the chilling-sensitive mutants we observed, mutations at one locus were characterized in detail. This gene, At1g45231, encodes an Arabidopsis ( ) trimethylguanosine synthase (TGS1), previously uncharacterized in the plant kingdom. We confirmed that Arabidopsis TGS1 is a functional ortholog of other trimethylguanosine synthases based both on its in vitro methyltransferase activity and on its ability to rescue the cold-growth inhibition of a mutant in vivo. While mutant plants grew normally at 22°C, their vegetative and reproductive growth was severely compromised under chilling conditions. When we transgenically expressed in the mutant plants, the chilling-sensitive phenotype was relieved, demonstrating that TGS1 is required for chilling tolerance.

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Title: Trimethylguanosine Synthase1 (TGS1) Is Essential for Chilling Tolerance
Creators: Jinpeng Gao - Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, Washington 99164-6340
James G Wallis - Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, Washington 99164-6340
Jeremy B Jewell - Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, Washington 99164-6340
John Browse - Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, Washington 99164-6340 jab@wsu.edu
Publication Details: Plant physiology (Bethesda), Vol.174(3), pp.1713-1727
Academic Unit: Plant Pathology, Department of; Biological Chemistry, Institute of
Publisher: United States
Identifiers: 99900548050101842
Language: English
Resource Type: Journal article