Journal article
Trpac1, a pH response transcription regulator, is involved in cellulase gene expression in Trichoderma reesei
Enzyme and microbial technology, Vol.67, pp.17-26
12/2014
Handle:
https://hdl.handle.net/2376/118076
PMID: 25442944
Abstract
•The pH regulation transcription factor Trpac1 was functional analyzed through target gene deletion strategy.•Trpac1 influences the cellulase production under neutral pH.•Trpac1 is involved in major cellulase gene expression on Avicel cellulose under neutral pH.•Trpac1 might influence cellulase genes expression by affecting the expression levels of potential regulator genes xyr1 and ace2 in T. reesei at neutral pH.
Fungi grow over a relatively wide pH range and adapt to extracellular pH through a genetic regulatory system mediated by a key component PacC, which is a pH transcription regulator. The cellulase production of the filamentous fungi Trichoderma reesei is sensitive to ambient pH. To investigate the connection between cellulase expression regulation and ambient pH, an ortholog of Aspergillus nidulans pacC, Trpac1, was identified and functionally characterized using a target gene deletion strategy. Deleting Trpac1 dramatically increased the cellulase production and the transcription levels of the major cellulase genes at neutral pH, which suggested Trpac1 is involved in the regulation of cellulase production. It was further observed that the expression levels of transcription factors xyr1 and ace2 also increased in the ΔTrpac1 mutant at neutral pH. In addition, the ΔTrpac1 mutant exhibited conidiation defects under neutral and alkaline pH. These results implied that Trpac1 in involved in growth and development process and cellulase gene expression in T. reesei.
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Details
- Title
- Trpac1, a pH response transcription regulator, is involved in cellulase gene expression in Trichoderma reesei
- Creators
- Ronglin He - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, ChinaLijuan Ma - College of Biotechnology, Tianjin University of Science and Technology, 13th Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, ChinaChen Li - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, ChinaWendi Jia - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, ChinaDemao Li - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, ChinaDongyuan Zhang - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, ChinaShulin Chen - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China
- Publication Details
- Enzyme and microbial technology, Vol.67, pp.17-26
- Publisher
- Elsevier Inc
- Identifiers
- 99900583057301842
- Language
- English
- Resource Type
- Journal article