Tumor necrosis factor α: Activity dependent expression and promotion of cortical column sleep in rats

L Churchill; D.M Rector; K Yasuda; C Fix; M.J Rojas; T Yasuda; J.M Krueger

doi:10.1016/j.neuroscience.2008.06.066

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Tumor necrosis factor α: Activity dependent expression and promotion of cortical column sleep in rats

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Tumor necrosis factor α: Activity dependent expression and promotion of cortical column sleep in rats

L Churchill, D.M Rector, K Yasuda, C Fix, M.J Rojas, T Yasuda and J.M Krueger

Neuroscience, Vol.156(1), pp.71-80

09/22/2008

DOI: https://doi.org/10.1016/j.neuroscience.2008.06.066

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https://hdl.handle.net/2376/114850

PMCID: PMC2654198

PMID: 18694809

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Abstract

evoked response potentials

cortical columns

cytokine

barrel field

surface evoked potentials

local sleep

Cortical surface evoked potentials (SEPs) are larger during sleep and characterize a sleep-like state in cortical columns. Since tumor necrosis factor alpha (TNF) may be involved in sleep regulation and is produced as a consequence of waking activity, we tested the hypothesis that direct application of TNF to the cortex will induce a sleep-like state within cortical columns and enhance SEP amplitudes. We found that microinjection of TNF onto the surface of the rat somatosensory cortex enhanced whisker stimulation-induced SEP amplitude relative to a control heat-inactivated TNF microinjection. We also determined if whisker stimulation enhanced endogenous TNF expression. TNF immunoreactivity (IR) was visualized after 2 h of deflection of a single whisker on each side. The number of TNF-IR cells increased in layers II–IV of the activated somatosensory barrel column. In two separate studies, unilateral deflection of multiple whiskers for 2 h increased the number of TNF-IR cells in layers II–V in columns that also exhibited enhanced cellular ongogene (Fos-IR). TNF-IR also colocalized with NeuN-IR suggesting that TNF expression was in neurons. Collectively these data are consistent with the hypotheses that TNF is produced in response to neural activity and in turn enhances the probability of a local sleep-like state as determined by increases in SEP amplitudes.

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Title: Tumor necrosis factor α: Activity dependent expression and promotion of cortical column sleep in rats
Creators: L Churchill - Department of VCAPP, Program in Neuroscience, College of Veterinary Medicine, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA
D.M Rector - Department of VCAPP, Program in Neuroscience, College of Veterinary Medicine, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA
K Yasuda - Hirosaki University School of Medicine, Department of Otorhinolaryngology, Hirosake 036-8216, Japan
C Fix - Department of VCAPP, Program in Neuroscience, College of Veterinary Medicine, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA
M.J Rojas - Department of VCAPP, Program in Neuroscience, College of Veterinary Medicine, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA
T Yasuda - Hirosaki University School of Medicine, Department of Anesthesiology, Hirosaki 036-8216, Japan
J.M Krueger - Department of VCAPP, Program in Neuroscience, College of Veterinary Medicine, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA
Publication Details: Neuroscience, Vol.156(1), pp.71-80
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: Elsevier Ltd
Identifiers: 99900547602601842
Language: English
Resource Type: Journal article