Journal article
Tumor necrosis factor enhances the sleep-like state and electrical stimulation induces a wake-like state in co-cultures of neurons and glia
The European journal of neuroscience, Vol.42(4), pp.2078-2090
08/2015
Handle:
https://hdl.handle.net/2376/115170
PMCID: PMC4540611
PMID: 26036796
Abstract
We characterise sleep-like states in cultured neurons and glia during development in vitro as well as after electrical stimulation, the addition of tumor necrosis factor alpha (TNF), and the combination of TNF plus electrical stimulation. We also characterise optogenetic stimulation-induced ATP release and neuronal interleukin-1 and TNF expression in vitro demonstrating the activity dependence of these putative sleep-regulatory substances. Action potential (AP) burstiness, expressed as the burstiness index (BI), synchronization of slow electrical potentials between recording electrodes (SYN), and slow wave (SW) power (0.25-3.75 Hz) determined using fast Fourier analyses emerged as network properties, maturing after 2 weeks in culture. Homologous in vivo measures are used to characterise sleep. Electrical stimulation reduced the BI, SYN and SW power values during and/or after the stimulus period. One day later, homeostasis was evident from rebounds of SYN and SW power values to above baseline levels; the magnitude of the rebound was stimulus pattern-dependent. The addition of TNF enhanced BI, SYN and SW power values, suggesting the induction of a deeper sleep-like state. Electrical stimulation reversed these TNF effects, suggesting the network state was more wake-like. The day after TNF plus electrical stimulation, the changes in SYN and SW power values were dependent upon the stimulus patterns the cells received the day before. We conclude that sleep and wake states in cultured in vitro networks can be controlled and they share molecular regulatory mechanisms with local in vivo networks. Further, sleep is an activity-dependent emergent local network property.
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Details
- Title
- Tumor necrosis factor enhances the sleep-like state and electrical stimulation induces a wake-like state in co-cultures of neurons and glia
- Creators
- Kathryn A Jewett - College of Medical Sciences and Washington State University, P.O. Box 1495, Spokane, 99202, WA, USAPing Taishi - College of Medical Sciences and Washington State University, P.O. Box 1495, Spokane, 99202, WA, USAParijat Sengupta - College of Medical Sciences and Washington State University, P.O. Box 1495, Spokane, 99202, WA, USASandip Roy - Department of Electrical Engineering, Washington State University, Pullman, WA, USAChristopher J Davis - College of Medical Sciences and Washington State University, P.O. Box 1495, Spokane, 99202, WA, USAJames M Krueger - College of Medical Sciences and Washington State University, P.O. Box 1495, Spokane, 99202, WA, USA
- Publication Details
- The European journal of neuroscience, Vol.42(4), pp.2078-2090
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of; Electrical Engineering and Computer Science, School of
- Publisher
- France
- Grant note
- R01 NS025378 / NINDS NIH HHS NS025378 / NINDS NIH HHS R01 HD036520 / NICHD NIH HHS
- Identifiers
- 99900547884201842
- Language
- English
- Resource Type
- Journal article