Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446

Cathryn A Hogarth; Ryan Evanoff; Debra Mitchell; Travis Kent; Christopher Small; John K Amory; Michael D Griswold

doi:10.1095/biolreprod.112.105346

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Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446

Journal article

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Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446

Cathryn A Hogarth, Ryan Evanoff, Debra Mitchell, Travis Kent, Christopher Small, John K Amory and Michael D Griswold

Biology of reproduction, Vol.88(2), pp.40-40

02/2013

DOI: https://doi.org/10.1095/biolreprod.112.105346

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https://hdl.handle.net/2376/109785

PMID: 23284139

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Abstract

Spermatogenesis - drug effects

Testis - metabolism

Testis - cytology

Male

Spermatogenesis - physiology

Animals

Testis - embryology

Tretinoin - metabolism

Cell Differentiation - drug effects

Diamines - pharmacology

Models, Animal

Mice

Cell Differentiation - physiology

The BDADs (bis-[dichloroacetyl]-diamines) are compounds that can inhibit spermatogenesis via blocking the metabolism of vitamin A. We utilized one specific BDAD, WIN 18,446, to manipulate the endogenous production of retinoic acid (RA) in the testis to further investigate the action of this compound on mammalian sperm production. Transient treatment of adult male mice with WIN 18,446 blocked spermatogonial differentiation and induced significant changes in the cycle of the seminiferous epithelium. WIN 18,446 treatment of neonatal mice also blocked spermatogonial differentiation and, followed by injection of RA, induced synchronous spermatogenesis in adulthood. The net result was pulsatile, rather than normal continuous, release of sperm from the seminiferous epithelium. This study describes a novel technique that can enrich for specific germ cell populations within the testis, representing a valuable new tool for studying spermatogenesis.

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Title: Turning a spermatogenic wave into a tsunami: synchronizing murine spermatogenesis using WIN 18,446
Creators: Cathryn A Hogarth - School of Molecular Biosciences and the Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA
Ryan Evanoff
Debra Mitchell
Travis Kent
Christopher Small
John K Amory
Michael D Griswold
Publication Details: Biology of reproduction, Vol.88(2), pp.40-40
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R01 HD010808 / NICHD NIH HHS U01 HD060408 / NICHD NIH HHS U54 42454 / PHS HHS HD 10808 / NICHD NIH HHS
Identifiers: 99900547419201842
Language: English
Resource Type: Journal article