Journal article
Two-Dimensional Fe-N-C Single-Atomic-Site Catalysts with Boosted Peroxidase-Like Activity for a Sensitive Immunoassay
Analytical chemistry (Washington), Vol.95(9), pp.4521-4528
03/07/2023
PMID: 36843270
Abstract
Single-atomic-site catalysts (SASCs) with peroxidase (POD)-like activities have been widely used in various sensing platforms, like the enzyme-linked immunosorbent assay (ELISA). Herein, a two-dimensional Fe-N-C-based SASC (2D Fe-SASC) is successfully synthesized with excellent POD-like activity (specific activity = 90.11 U/mg) and is used to design the ELISA for herbicide detection. The 2D structure of Fe-SASC enables the exposure of numerous single atomic active sites on the surface as well as boosts the POD-like activity, thereby enhancing the sensing performance. 2D Fe-SASC is assembled into competitive ELISA kit, which achieves an excellent detection performance for 2,4-dichlorophenoxyacetic acid (2,4-D). Fe-SASC has great potential in replacing high-cost natural enzymes and working on various advanced sensing platforms with high sensitivity for the detection of various target biomarkers.
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Details
- Title
- Two-Dimensional Fe-N-C Single-Atomic-Site Catalysts with Boosted Peroxidase-Like Activity for a Sensitive Immunoassay
- Creators
- Zhaoyuan Lyu - Washington State UniversityShichao Ding - Washington State UniversityLingzhe Fang - Northern Illinois UniversityXin Li - Washington State UniversityTao Li - Northern Illinois UniversityMingjie Xu - University of California, IrvineXiaoqing Pan - University of California, IrvineWenlei Zhu - Washington State UniversityYang Zhou - Washington State UniversityDan Du - DL ADV Tech, Pullman, WA 99163 USAYuehe Lin - Washington State University
- Publication Details
- Analytical chemistry (Washington), Vol.95(9), pp.4521-4528
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 8
- Grant note
- DMR-2011967 / National Science Foundation through the UC Irvine Materials Research Science and Engineering Center DE-AC02-06CH11357 / U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences; United States Department of Energy (DOE) 1R43ES031885 - 01 / National Institute of Environmental Health Sciences of the U.S. National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99901227636801842
- Language
- English
- Resource Type
- Journal article