Journal article
Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of Erhualian pigs
Animal genetics, Vol.44(4), pp.458-462
08/2013
Handle:
https://hdl.handle.net/2376/110941
PMID: 23402337
Abstract
Summary
This article is the first to report the significant effect of PPARG promoter polymorphisms on the expression and intramuscular fat (IMF) content in the longissimus dorsi (LD) muscle of Erhualian pigs. Polymorphic sites for the PPARG upstream transcriptional regulatory region were scanned by direct sequencing of two DNA pools consisting of individuals with significantly different IMF content (P < 0.05). Two SNPs (c.‐1633C>T and c.‐1572G>A) were identified, with different allele distribution between these two groups. A total of 66 Erhualian pigs and 24 Yorkshire × Large White (Y × L) pigs were genotyped for these two SNPs using PCR‐SSCP (single‐strand conformation polymorphism), which exhibited that only the T‐A and C‐G haplotypes existed. The T‐A type promoter was found to have higher activity than G‐C type promoter for PPARG gene transcription in the induced preadipocytes using luciferase assay. In addition, the frequency of the T‐A type in Erhualian pigs was much higher than that in the Y × L pigs (35.6% vs. 8.3%), and in fact, no T‐A/T‐A samples were found in Y × L pigs. Therefore, we conclude that the T‐A haplotype of PPARG is a desirable form that might contribute to the relatively higher IMF content in Erhualian pigs.
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Details
- Title
- Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of Erhualian pigs
- Creators
- H Wang - Nanjing Agricultural UniversityK Xiong - Nanjing Agricultural UniversityW Sun - Nanjing Agricultural UniversityY Fu - Nanjing Agricultural UniversityZ Jiang - Washington State UniversityD Yu - Nanjing Agricultural UniversityH Liu - Nanjing Agricultural UniversityJ Chen - Nanjing Agricultural University
- Publication Details
- Animal genetics, Vol.44(4), pp.458-462
- Academic Unit
- Animal Sciences, Department of
- Number of pages
- 5
- Grant note
- Fundamental Research Funds for the Central Universities (No. KYZ201113) National Natural Science Foundation of China (No. 31272423)
- Identifiers
- 99900547378501842
- Language
- English
- Resource Type
- Journal article