Journal article
Two-dimensional liquid chromatography–tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine circovirus type 2
Journal of proteomics, Vol.79, pp.72-86
02/21/2013
Handle:
https://hdl.handle.net/2376/104846
PMID: 23238063
Abstract
Porcine circovirus type 2 (PCV2) has been identified as the essential causal agent of postweaning multisystemic wasting syndrome, which has spread worldwide. Monocyte/macrophage lineage cells are the major target cells of PCV2. To discover cellular protein responses of pulmonary alveolar macrophages (PAMs) to PCV2 infection, two-dimensional liquid chromatography–tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the proteins that were differentially expressed in PAMs from the PCV2-infected group compared to the uninfected control group. A total of 145 cellular proteins in PAMs that were significantly altered at different time periods post-infection were identified. These differentially expressed proteins were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. The high number of differentially expressed proteins identified should be very useful to elucidate the mechanism of replication and pathogenesis of PCV2 in the future.
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► The cellular protein profile of PCV2-infected PAMs was investigated by iTRAQ. ► Some significantly regulated proteins and pathways were related to pathological responses of PCV2 infection. ► The data will be helpful for defining the molecular mechanism of replication and pathogenesis of PCV2.
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Details
- Title
- Two-dimensional liquid chromatography–tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine circovirus type 2
- Creators
- Jie Liu - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,ChinaJuan Bai - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,ChinaQi Lu - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,ChinaLili Zhang - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,ChinaZhihua Jiang - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351,USAJennifer J Michal - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351,USAQingdong He - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,ChinaPing Jiang - Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China
- Publication Details
- Journal of proteomics, Vol.79, pp.72-86
- Academic Unit
- Animal Sciences, Department of; Languages, Cultures, and Race, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546868301842
- Language
- English
- Resource Type
- Journal article