Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice

Ming-Han Tong; Debra Ann Mitchell; Samantha Dawn McGowan; Ryan Evanoff; Michael D Griswold

doi:10.1095/biolreprod.111.096313

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Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice

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Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice

Ming-Han Tong, Debra Ann Mitchell, Samantha Dawn McGowan, Ryan Evanoff and Michael D Griswold

Biology of reproduction, Vol.86(3), pp.72-72

03/2012

DOI: https://doi.org/10.1095/biolreprod.111.096313

Handle:

https://hdl.handle.net/2376/101490

PMID: 22116806

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Abstract

Spermatogenesis - genetics

Testis - cytology

Mice, Inbred C57BL

Male

Signal Transduction - genetics

Down-Regulation - drug effects

Testis - physiology

Cell Differentiation - genetics

Spermatogenesis - physiology

Animals

Signal Transduction - physiology

Mice

MicroRNAs - genetics

MicroRNAs - physiology

Tretinoin - pharmacology

Increasing evidence indicates that microRNAs (miRNAs) may be critical players in spermatogenesis. The miRNA expression profiles of THY1(+)-enriched undifferentiated spermatogonia were characterized, and members of Mir-17-92 (Mirc1) and its paralog Mir-106b-25 (Mirc3) clusters are significantly downregulated during retinoic acid-induced spermatogonial differentiation, both in vitro and in vivo. The repression of microRNA clusters Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3) by retinoic acid in turn potentially upregulates the expression of Bim, Kit, Socs3, and Stat3. The male germ cell-specific Mir-17-92 (Mirc1) knockout mice exhibit small testes, a lower number of epididymal sperm, and mild defect in spermatogenesis. Absence of Mir-17-92 (Mirc1) in male germ cells dramatically increases expression of Mir-106b-25 (Mirc3) cluster miRNAs in the germ cells. These results suggest that Mir-17-92 (Mirc1) cluster and Mir-106b-25 (Mirc3) cluster miRNAs possibly functionally cooperate in regulating spermatogonial development.

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Title: Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice
Creators: Ming-Han Tong - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
Debra Ann Mitchell
Samantha Dawn McGowan
Ryan Evanoff
Michael D Griswold
Publication Details: Biology of reproduction, Vol.86(3), pp.72-72
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R37 HD010808 / NICHD NIH HHS R21 HD067775 / NICHD NIH HHS HD06777 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS R21 HD067775-01A1 / NICHD NIH HHS R21 HD067775-02 / NICHD NIH HHS HD10808 / NICHD NIH HHS
Identifiers: 99900546515001842
Language: English
Resource Type: Journal article