Journal article
Two variants of MutS homolog hMSH5: Prevalence in humans and effects on protein interaction
Biochemical and biophysical research communications, Vol.332(2), pp.524-532
2005
Handle:
https://hdl.handle.net/2376/116348
PMID: 15907804
Abstract
MSH5 is known to play functional roles in an array of cellular processes such as DNA damage response and meiotic homologous recombination. Here, we report the characterization of an hMSH5 splicing variant (hMSH5sv) that resulted from the retention of the last 51
bp of
hMSH5 intron 6, in which the encoded 17-amino acid insertion between codons 179 and 180 does not compromise its capability to interact with hMSH4. We have also identified an hMSH5 polymorphism (C84T) that altered codon 29 of the
hMSH5 gene resulting in a proline-to-serine change (P29S). The interaction domains of hMSH4 and hMSH5 have also been resolved. The P29S alteration is located within the interacting domain and leads to a weakened protein interaction with hMSH4. Together, our present study revealed the existence of two forms of hMSH5 variants in human cells. The different properties associated with these two hMSH5 variants underscore the potential functional diversity of the human
hMSH5 gene.
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Details
- Title
- Two variants of MutS homolog hMSH5: Prevalence in humans and effects on protein interaction
- Creators
- Wei Yi - School of Molecular Biosciences and Center for Reproductive Biology, Post Box 644660, Washington State University, Pullman, WA 99164-4660, USAXiling Wu - School of Molecular Biosciences and Center for Reproductive Biology, Post Box 644660, Washington State University, Pullman, WA 99164-4660, USATai-Hsien Lee - School of Molecular Biosciences and Center for Reproductive Biology, Post Box 644660, Washington State University, Pullman, WA 99164-4660, USANorman A Doggett - Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USAChengtao Her - School of Molecular Biosciences and Center for Reproductive Biology, Post Box 644660, Washington State University, Pullman, WA 99164-4660, USA
- Publication Details
- Biochemical and biophysical research communications, Vol.332(2), pp.524-532
- Academic Unit
- Horticulture, Department of; Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547690201842
- Language
- English
- Resource Type
- Journal article