Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection

W P Cheevers; J C Beyer; D P Knowles

doi:10.1128/JVI.71.8.6259-6263.1997

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Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection

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Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection

W P Cheevers, J C Beyer and D P Knowles

Journal of virology, Vol.71(8), pp.6259-6263

08/1997

DOI: https://doi.org/10.1128/JVI.71.8.6259-6263.1997

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https://hdl.handle.net/2376/106863

PMCID: PMC191895

PMID: 9223529

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https://doi.org/10.1128/JVI.71.8.6259-6263.1997View

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Abstract

Arthritis-Encephalitis Virus, Caprine

Interleukin-2 - genetics

Viral Envelope Proteins - physiology

Animals

Goats

Lymphocyte Activation

Lentivirus Infections - immunology

RNA, Messenger - analysis

T-Lymphocytes - immunology

CD4-Positive T-Lymphocytes

Interferon-gamma - genetics

Interleukin-4 - genetics

Peripheral blood mononuclear cells (PBMC) from Saanen goats experimentally infected with the lentivirus caprine arthritis-encephalitis virus (CAEV) were evaluated by semiquantitative reverse transcriptase PCR for gamma interferon (IFN-gamma), interleukin-4 (IL-4), and IL-2 gene expression following in vitro stimulation with purified CAEV gp135 surface protein (SU). Studies examined three goats with chronic arthritis and four clinically asymptomatic goats at 5 years postinfection. SU-responsive IFN-gamma mRNA-positive cells and IL-4 mRNA-positive cells in PBMC from infected goats reflected differences in lymphokine balance associated with disease status. IFN-gamma mRNA-positive cells were dominant in PBMC from asymptomatic goats, whereas SU-responsive IL-4 mRNA-positive cells were dominant in PBMC from goats with arthritis. IL-2 gene expression was not responsive to SU stimulation of PBMC from either asymptomatic or arthritic goats. Lymphokine mRNA profiles in SU-stimulated PBMC were dependent on the presence of CD4+ T lymphocytes. The results indicate that asymptomatic goats have a dominant population of CAEV SU-reactive T-helper 1 (Th1)-like lymphocytes in PBMC whereas goats with clinical arthritis have a dominant population of SU-reactive Th2-like lymphocytes.

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Title: Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection
Creators: W P Cheevers - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-6040, USA. cheevers@vetmed.wsu.edu
J C Beyer
D P Knowles
Publication Details: Journal of virology, Vol.71(8), pp.6259-6263
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: K08 AI01384 / NIAID NIH HHS R01 AR43718 / NIAMS NIH HHS
Identifiers: 99900546888101842
Language: English
Resource Type: Journal article