Journal article
Type I adenylyl cyclase functions as a coincidence detector for control of cyclic AMP response element-mediated transcription: synergistic regulation of transcription by Ca2+ and isoproterenol
Molecular and cellular biology, Vol.14(12), pp.8272-8281
12/1994
Handle:
https://hdl.handle.net/2376/109453
PMCID: PMC359366
PMID: 7969163
Abstract
Studies carried out with mammals and invertebrates suggest that Ca(2+)-sensitive adenylyl cyclases may be important for neuroplasticity. Long-term potentiation in the hippocampus requires increases in intracellular Ca2+ which are accompanied by elevated cyclic AMP (cAMP). Furthermore, activation of cAMP-dependent protein kinase is required for the late stage of long-term potentiation in the CA1 region of the hippocampus, which is also sensitive to inhibitors of transcription. Therefore, some forms of synaptic plasticity may require coordinate regulation of transcription by Ca2+ and cAMP. In this study, we demonstrate that the expression of type I adenylyl cyclase in HEK-293 cells allows Ca2+ to stimulate reporter gene activity mediated through the cAMP response element. Furthermore, simultaneous activation by Ca2+ and isoproterenol caused synergistic stimulation of transcription in HEK-293 cells and cultured neurons. We propose that Ca2+ and neurotransmitter stimulation of type I adenylyl cyclase may play a role in synaptic plasticity by generating optimal cAMP signals for regulation of transcription.
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Details
- Title
- Type I adenylyl cyclase functions as a coincidence detector for control of cyclic AMP response element-mediated transcription: synergistic regulation of transcription by Ca2+ and isoproterenol
- Creators
- S Impey - Department of Pharmacology, University of Washington, Seattle 98195G Wayman - Department of Pharmacology, University of Washington, Seattle 98195Z Wu - Department of Pharmacology, University of Washington, Seattle 98195D R Storm - Department of Pharmacology, University of Washington, Seattle 98195
- Publication Details
- Molecular and cellular biology, Vol.14(12), pp.8272-8281
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547280301842
- Language
- English
- Resource Type
- Journal article