Journal article
Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines
Microbiology (Society for General Microbiology), Vol.155(Pt 3), pp.837-851
03/2009
Handle:
https://hdl.handle.net/2376/114096
PMID: 19246755
Abstract
The Vibrio parahaemolyticus type III secretion system 1 (T3SS1) induces cytotoxicity in mammalian epithelial cells. We characterized the cell death phenotype in both epithelial (HeLa) and monocytic (U937) cell lines following infection with V. parahaemolyticus. Using a combination of the wild-type strain and gene knockouts, we confirmed that V. parahaemolyticus strain NY-4 was able to induce cell death in both cell lines via a T3SS1-dependent mechanism. Bacterial contact, but not internalization, was required for T3SS1-induced cytotoxicity. The mechanism of cell death involves formation of a pore structure on the surface of infected HeLa and U937 cells, as demonstrated by cellular swelling, uptake of cell membrane-impermeable dye and protection of cytotoxicity by osmoprotectant (PEG3350). Western blot analysis showed that poly ADP ribose polymerase (PARP) was not cleaved and remained in its full-length active form. This result was evident for seven different V. parahaemolyticus strains. V. parahaemolyticus-induced cytotoxicity was not inhibited by addition of the pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) or the caspase-1 inhibitor N-acetyl-tyrosyl-valyl-alanyl-aspartyl-aldehyde (Ac-YVAD-CHO); thus, caspases were not involved in T3SS1-induced cytotoxicity. DNA fragmentation was not evident following infection and autophagic vacuoles were not observed after monodansylcadaverine staining. We conclude that T3SS1 of V. parahaemolyticus strain NY-4 induces a host cell death primarily via oncosis rather than apoptosis, pyroptosis or autophagy.
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Details
- Title
- Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines
- Creators
- Xiaohui Zhou - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USAMichael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, WA, USADouglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA
- Publication Details
- Microbiology (Society for General Microbiology), Vol.155(Pt 3), pp.837-851
- Academic Unit
- Paul G. Allen School for Global Animal Health; Molecular Biosciences, School of
- Publisher
- England
- Grant note
- N01-AI-30055 / NIAID NIH HHS
- Identifiers
- 99900548171901842
- Language
- English
- Resource Type
- Journal article