UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression

C M Steber; R E Esposito

doi:10.1073/pnas.92.26.12490

Back

UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression

Journal article

Open access

UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression

C M Steber and R E Esposito

Proceedings of the National Academy of Sciences - PNAS, Vol.92(26), pp.12490-12494

12/19/1995

DOI: https://doi.org/10.1073/pnas.92.26.12490

Handle:

https://hdl.handle.net/2376/103164

PMCID: PMC40383

PMID: 8618927

Files and links (1)

url

https://doi.org/10.1073/pnas.92.26.12490View

Published (Version of record) Open

Abstract

Gene Expression Regulation, Fungal

Promoter Regions, Genetic

Saccharomyces cerevisiae - physiology

Saccharomyces cerevisiae - genetics

Repressor Proteins

Single-Strand Specific DNA and RNA Endonucleases

DNA Replication

Meiosis

Fungal Proteins - genetics

Transcription Factors - genetics

DNA-Binding Proteins - genetics

DNA-Binding Proteins - metabolism

Saccharomyces cerevisiae - cytology

Transcription Factors - metabolism

DNA, Fungal - biosynthesis

Flow Cytometry

Time Factors

Recombination, Genetic

Spores, Fungal

DNA, Fungal - metabolism

Saccharomyces cerevisiae Proteins

Genes, Fungal

Kinetics

The UME6 gene of Saccharomyces cerevisiae was identified as a mitotic repressor of early meiosis-specific gene expression. It encodes a Zn2Cys6 DNA-binding protein which binds to URS1, a promoter element needed for both mitotic repression and meiotic induction of early meiotic genes. This paper demonstrates that a complete deletion of UME6 causes not only vegetative derepression of early meiotic genes during vegetative growth but also a significant reduction in induction of meiosis-specific genes, accompanied by a severe defect in meiotic progression. After initiating premeiotic DNA synthesis the vast majority of cells (approximately 85%) become arrested in prophase and fail to execute recombination; a minority of cells (approximately 15%) complete recombination and meiosis I, and half of these form asci. Quantitative analysis of the same early meiotic transcripts that are vegetatively derepressed in the ume6 mutant, SPO11, SPO13, IME2, and SPO1, indicates a low level of induction in meiosis above their vegetative derepressed levels. In addition, the expression of later meiotic transcripts, SPS2 and DIT1, is significantly delayed and reduced. The expression pattern of early meiotic genes in ume6-deleted cells is strikingly similar to that of early meiotic genes with promoter mutations in URS1. These results support the view that UME6 and URS1 are part of a developmental switch that controls both vegetative repression and meiotic induction of meiosis-specific genes.

Metrics

10 Record Views

See more details

Details

Title: UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression
Creators: C M Steber - Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637, USA
R E Esposito
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.92(26), pp.12490-12494
Academic Unit: Crop and Soil Sciences, Department of
Publisher: United States
Grant note: GM29182 / NIGMS NIH HHS T32GM07197 / NIGMS NIH HHS
Identifiers: 99900546641801842
Language: English
Resource Type: Journal article