Journal article
Unique features of bovine lymphocytes exposed to a staphylococcal enterotoxin
Journal of veterinary science (Suwŏn-si, Korea), Vol.7(3), pp.233-239
09/2006
Handle:
https://hdl.handle.net/2376/113422
PMCID: PMC3242122
PMID: 16871017
Abstract
We previously demonstrated that stimulation of bovine peripheral blood mononuclear cells (PBMCs) with staphylococcal enterotoxin C (SEC), led to an inversion of the CD4
+
:CD8
+
T cell ratio and generation of an atypical CD8
+
T cell subpopulation expressing CD26. In the present study, we examined T cell apoptosis and proliferation profiles of PBMC subpopulations in cultures stimulated with SEC. Unlike when stimulated with concanavalin A, nucleic acid synthesis in bovine PBMC cultures stimulated with SEC was low during the first four days but increased greatly on day 5. In contrast, nucleic acid synthesis in human PBMC cultures stimulated with SEC increased continuously. To investigate the mechanism of delayed bovine T cell proliferation, various cell phenotypes were monitored. The inversion of the bovine CD4
+
:CD8
+
T cell ratio in PBMC cultures stimulated by SEC was associated with higher proliferation and lower apoptosis of CD8
+
T cells compared to CD4
+
T cells. The mRNA levels for interleukin (IL)-4 and IL-13 were sustained over 4 days but IL-12 mRNA levels dropped to background on day 2. These data suggest that SEC induces a prolonged Th-2-biased microenvironment, and together with the inversion of the bovine CD4
+
:CD8
+
T cell ratios in bovine PBMC cultures with SEC, may in part explain the inability of the mammary immune system to establish an effective response to
Staphylococcus aureus
infections.
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Details
- Title
- Unique features of bovine lymphocytes exposed to a staphylococcal enterotoxin
- Creators
- Yong Ho Park - Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, KoreaSang Un Lee - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USAWitold A Ferens - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USASparrow Samuels - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USAWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USALawrence K Fox - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USAJong Sam Ahn - Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USAKeun Seok Seo - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USAByoung Sun Chang - Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, KoreaSun Young Hwang - Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, KoreaGregory A Bohach - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA
- Publication Details
- Journal of veterinary science (Suwŏn-si, Korea), Vol.7(3), pp.233-239
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- The Korean Society of Veterinary Science
- Identifiers
- 99900547629901842
- Language
- English
- Resource Type
- Journal article