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CC BY V4.0, Open Access
Journal article
Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge
BMC evolutionary biology, Vol.10(1), pp.296-296
2010
Handle:
https://hdl.handle.net/2376/106059
PMCID: PMC2955719
PMID: 20920202
Abstract
Background: In most protein-coding genes, greater sequence variation is observed in noncoding regions (introns and untranslated regions) than in coding regions due to selective constraints. During characterization of genes and transcripts encoding small secreted salivary gland proteins (SSSGPs) from the Hessian fly, we found exactly the opposite pattern of conservation in several families of genes: the non-coding regions were highly conserved, but the coding regions were highly variable. Results: Seven genes from the SSSGP-1 family are clustered as one inverted and six tandem repeats within a 15 kb region of the genome. Except for SSSGP-1A2, a gene that encodes a protein identical to that encoded by SSSGP-1A1, the other six genes consist of a highly diversified, mature protein-coding region as well as highly conserved regions including the promoter, 5'-and 3'-UTRs, a signal peptide coding region, and an intron. This unusual pattern of highly diversified coding regions coupled with highly conserved regions in the rest of the gene was also observed in several other groups of SSSGP-encoding genes or cDNAs. The unusual conservation pattern was also found in some of the SSSGP cDNAs from the Asian rice gall midge, but not from the orange wheat blossom midge. Strong positive selection was one of the forces driving for diversification whereas concerted homogenization was likely a mechanism for sequence conservation.
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Details
- Title
- Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge
- Creators
- Ming-Shun Chen - Kansas State UniversityXuming Liu - Kansas State UniversityZiheng Yang - University College LondonHuixian Zhao - Northwest A&F UniversityRichard H Shukle - USDA-ARS and Department of Entomology, Purdue University, West Lafayette, IN 47907, USAJeffrey J Stuart - USDA-ARS and Department of Entomology, Purdue University, West Lafayette, IN 47907, USAScot Hulbert - Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA
- Publication Details
- BMC evolutionary biology, Vol.10(1), pp.296-296
- Academic Unit
- Department of Plant Pathology
- Publisher
- BioMed Central
- Identifiers
- 99900546615901842
- Language
- English
- Resource Type
- Journal article