Journal article
Use of antisense oligodeoxynucleotide to determine δ-opioid receptor involvement in [ d-Ala 2]deltorphin II-induced locomotor hyperactivity
Life sciences (1973), Vol.59(4), pp.PL69-PL73
1996
Handle:
https://hdl.handle.net/2376/100428
PMID: 8761010
Abstract
Intracerebroventricularly (i.c.v.)-administered [
d-Ala
2]deltorphin II (20 μg) produced a marked locomotor hyperactivity in male ICR mice. The locomotor hyperactivity induced in response to i.c.v. [
d-Ala
2]deltorphin II (20 μg) was suppressed by pretreatment with naltriben (NTB, 10 μg) but not 7-benzylidene naltrexone (BNTX, 1 μg) and
d-Phe-Cys-Tyr-
d-Try-Orn-Thr-Phe-Thr-NH
2 (CTOP, 100 ng). The influence of antisense oligodeoxynucleotide to δ-opioid receptor mRNA (δ-AS oligo) or a mismatch oligodeoxynucleotide (MM oligo) on the locomotor hyperactivity induced by [
d-Ala
2]deltorphin II was determined. Groups of mice pretreated i.c.v. with δ-AS oligo (1 μg), MM oligo (1 μg) or saline (4 μl) once a day for 3 days, were injected i.c.v. [
d-Ala
2]deltorphin II (10 or 20 μg) and the locomotor response to [
d-Ala
2]deltorphin II was measured. The locomotor hyperactivity of i.c.v. [
d-Ala
2]deltorphin II (10 or 20 μg) were significantly suppressed by i.c.v. pretreatment with δ-AS oligo but not MM oligo. The present results indicate that pretreatment with δ-AS oligo suppresses mouse locomotor hyperactivity produced by stimulation of
δ
2-opioid receptors in the brain.
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Details
- Title
- Use of antisense oligodeoxynucleotide to determine δ-opioid receptor involvement in [ d-Ala 2]deltorphin II-induced locomotor hyperactivity
- Creators
- Hirokazu Mizoguchi - Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USAMinoru Narita - Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USAHiroshi Nagase - Basic Research Laboratories, Toray Industries Inc., Kamakura 248, JapanTsutomu Suzuki - Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo 142, JapanRaymond M Quock - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USALeon F Tseng - Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
- Publication Details
- Life sciences (1973), Vol.59(4), pp.PL69-PL73
- Publisher
- Elsevier Inc
- Identifiers
- 99900546688701842
- Language
- English
- Resource Type
- Journal article