Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

William C Davis; Mary Jo Hamilton

doi:10.4142/jvs.2008.9.1.51

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Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

Journal article

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Peer reviewed

Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

William C Davis and Mary Jo Hamilton

Journal of veterinary science (Suwŏn-si, Korea), Vol.9(1), pp.51-66

03/2008

DOI: https://doi.org/10.4142/jvs.2008.9.1.51

Handle:

https://hdl.handle.net/2376/116007

PMCID: PMC2839113

PMID: 18296889

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Abstract

Rabbits

B-Lymphocytes - cytology

Granulocytes - cytology

Epitopes - metabolism

Monocytes - cytology

Gene Expression Regulation

Monocytes - metabolism

Epitopes - genetics

Basophils - cytology

Leukocytes - immunology

Animals

Flow Cytometry

T-Lymphocytes - cytology

T-Lymphocytes - metabolism

Antigens, Differentiation - metabolism

Mice

Granulocytes - metabolism

Basophils - metabolism

Leukocytes - metabolism

Antibodies, Monoclonal - immunology

B-Lymphocytes - metabolism

Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of the Eighth Human Leukocyte Differentiation Workshop yielded 7 additional mAbs. Screening of mAbs generated from mice immunized with leukocytes from rabbit thymus or spleen or concanavalin A activated peripheral blood and/or spleen lymphocytes has yielded 42 mAbs that recognize species restricted epitopes expressed on one or more lineages of leukocytes. Screening of the anti-rabbit mAbs against leukocytes from other species yielded one additional mAb. The studies show that screening of existing sets of mAbs for reactivity with rabbit LDM will not be productive and that a direct approach will be needed to develop mAbs for research in rabbits. The flow cytometric approach we developed to screen for mAbs of interest offers a way for individual laboratories to identify and characterize mAbs to LDM in rabbits and other species. A web-based program we developed provides a source of information that will facilitate analysis. It contains a searchable data base on known CD molecules and a data base on mAbs, known to react with LDM in one or more species of artiodactyla, equidae, carnivora, and or lagomorpha.

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Title: Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules
Creators: William C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA. davisw@vetmed.wsu.edu
Mary Jo Hamilton
Publication Details: Journal of veterinary science (Suwŏn-si, Korea), Vol.9(1), pp.51-66
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: Korea (South)
Identifiers: 99900547996101842
Language: English
Resource Type: Journal article